Anti-CD40L/CD40LG Antibody Picoband™

Boster Bio Anti-CD40L/CD40LG Antibody Picoband™ catalog # A01114-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A01114-3
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-CD40L/CD40LG Antibody Picoband™

See all CD40 Ligand/TNFSF5 products

SKU/Catalog Number

A01114-3

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD40L/CD40LG Antibody Picoband™ catalog # A01114-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD40L/CD40LG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01114-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence of human CD40L/CD40LG (KEETKKENSFEMQKGDQNPQIAAHVISEA).

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A01114-3 is reactive to CD40LG in Human, Mouse, Rat

Applications

A01114-3 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For CD40LG (Source: Uniprot.Org, NCBI)

Uniprot ID

P29965

Gene ID

959

Gene Name

CD40LG

Full Name

CD40 ligand

Weight

29.274kDa

Superfamily

tumor necrosis factor family

Alternative Names

CD154 antigen; CD154; CD40 antigen ligand; CD40 Ligand; CD40L; CD40-L; CD40LG; CD40LIGM; gp39; hCD40L; HIGM1; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TNFSF5IMD3; TRAP; TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5 CD40LG CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L CD40 ligand CD40 ligand|CD40 antigen ligand|CD40-L|T-B cell-activating molecule|T-cell antigen Gp39|TNF-related activation protein|tumor necrosis factor (ligand) superfamily member 5

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD40LG, check out the CD40LG Infographic

CD40LG infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD40LG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A01114-3 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Yang G, Fang Z, Liu Y, Zhang H, Shi X, Ji Q, Lin Q, Lin R. Evid Based Complement Alternat Med. 2011;2011:930324. Doi: 10.1093/Ecam/Nep013. Epub 2011 Jun 5. Protective Effects Of Chinese Traditional Medicine Buyang Huanwu Decoction On Myocardial In...

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15 Customer Q&As for Anti-CD40L/CD40LG Antibody Picoband™

Question

Here is the WB image, lot number and protocol we used for blood using anti-CD40L/CD40LG antibody A01114-3. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-09

Question

I have a question about product A01114-3, anti-CD40L/CD40LG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01114-3 anti-CD40L/CD40LG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-28

Question

I was wanting to use your anti-CD40L/CD40LG antibody for WB for mouse blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse blood identification?

Verified Customer

Verified customer

Asked: 2020-01-17

Answer

It shows on the product datasheet, A01114-3 anti-CD40L/CD40LG antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-17

Question

I see that the anti-CD40L/CD40LG antibody A01114-3 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-30

Question

Is there a BSA free version of anti-CD40L/CD40LG antibody A01114-3 available?

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD40L/CD40LG antibody A01114-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-07

Question

Is this A01114-3 anti-CD40L/CD40LG antibody reactive to the isotypes of CD40LG?

F. Johnson

Verified customer

Asked: 2019-06-26

Answer

The immunogen of A01114-3 anti-CD40L/CD40LG antibody is A synthetic peptide corresponding to a sequence of human CD40L/CD40LG(KEETKKENSFEMQKGDQNPQIAAHVISEA). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-26

Question

I am looking for using your anti-CD40L/CD40LG antibody for inflammatory response studies. Has this antibody been tested with western blotting on mouse thymus tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-05-30

Answer

Thanks for your inquiry. This A01114-3 anti-CD40L/CD40LG antibody is tested on rat stomach tissue, liver tissue, mouse thymus tissue, spleen tissue. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-05-30

Question

Would anti-CD40L/CD40LG antibody A01114-3 work for WB with blood?

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

According to the expression profile of blood, CD40LG is highly expressed in blood. So, it is likely that anti-CD40L/CD40LG antibody A01114-3 will work for WB with blood.

Boster Scientific Support

Answered: 2019-05-23

Question

We have seen staining in rat blood. What should we do? Is anti-CD40L/CD40LG antibody supposed to stain blood positively?

Verified Customer

Verified customer

Asked: 2018-10-09

Answer

Based on literature blood does express CD40LG. Based on Uniprot.org, CD40LG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have CD40LG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-10-09

Question

We are currently using anti-CD40L/CD40LG antibody A01114-3 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2018-03-05

Answer

The anti-CD40L/CD40LG antibody (A01114-3) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-03-05

Question

My boss were well pleased with the WB result of your anti-CD40L/CD40LG antibody. However we have been able to see positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is CD40LG supposed to be expressed?

J. Brown

Verified customer

Asked: 2017-12-22

Answer

According to literature, leukocyte does express CD40LG. Generally CD40LG expresses in cell membrane. Regarding which tissues have CD40LG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-12-22

Question

Would A01114-3 anti-CD40L/CD40LG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-10-19

Answer

You can see on the product datasheet, A01114-3 anti-CD40L/CD40LG antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-10-19

Question

My lab would like to test anti-CD40L/CD40LG antibody A01114-3 on mouse blood for research purposes, then I may be interested in using anti-CD40L/CD40LG antibody A01114-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

M. Thomas

Verified customer

Asked: 2016-07-18

Answer

The products we sell, including anti-CD40L/CD40LG antibody A01114-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-07-18

Question

Is a blocking peptide available for product anti-CD40L/CD40LG antibody (A01114-3)?

C. Evans

Verified customer

Asked: 2015-07-22

Answer

We do provide the blocking peptide for product anti-CD40L/CD40LG antibody (A01114-3). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-07-22

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-CD40L/CD40LG antibody A01114-3. Let me know if you need anything else.

N. Brown

Verified customer

Asked: 2014-10-01

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-10-01

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