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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-CD40L/CD40LG Antibody Picoband™
Boster Bio Anti-CD40L/CD40LG Antibody Picoband™ catalog # A01114-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD40L/CD40LG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01114-3)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence of human CD40L/CD40LG (KEETKKENSFEMQKGDQNPQIAAHVISEA).
No cross reactivity with other proteins.
A01114-3 is reactive to CD40LG in Human, Mouse, Rat
A01114-3 is guaranteed for WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD40LG using anti-CD40LG antibody (A01114-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40LG antigen affinity purified polyclonal antibody (Catalog # A01114-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40LG at approximately 36KD. The expected band size for CD40LG is at 29KD.
Gene/Protein Information For CD40LG (Source: Uniprot.Org, NCBI)
tumor necrosis factor family
CD154 antigen; CD154; CD40 antigen ligand; CD40 Ligand; CD40L; CD40-L; CD40LG; CD40LIGM; gp39; hCD40L; HIGM1; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TNFSF5IMD3; TRAP; TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5 CD40LG CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L CD40 ligand CD40 ligand|CD40 antigen ligand|CD40-L|T-B cell-activating molecule|T-cell antigen Gp39|TNF-related activation protein|tumor necrosis factor (ligand) superfamily member 5*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CD40LG, check out the CD40LG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD40LG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A01114-3 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yang G, Fang Z, Liu Y, Zhang H, Shi X, Ji Q, Lin Q, Lin R. Evid Based Complement Alternat Med. 2011;2011:930324. Doi: 10.1093/Ecam/Nep013. Epub 2011 Jun 5. Protective Effects Of Chinese Traditional Medicine Buyang Huanwu Decoction On Myocardial In...
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