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Product Info Summary
| SKU: | M01102 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD41 ITGA2B Rabbit Monoclonal Antibody
SKU/Catalog Number
M01102
BM5058 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD41 ITGA2B Rabbit Monoclonal Antibody catalog # M01102. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD41 ITGA2B Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01102)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAGH-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD41
Reactive Species
M01102 is reactive to ITGA2B in Human
Observed Molecular Weight
130 kDa
Calculated molecular weight
113.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01102 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: human HEL whole cell
IHC: Rat pancreas tissue, Human squamous carcinoma tissue, Human placenta tissue
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Rat pancreas, using the Antibody at 1:50 dilution.
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Western blot analysis of ITGA2B using anti-ITGA2B antibody (M01102).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA2B antigen affinity purified monoclonal antibody (Catalog # M01102) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGA2B at approximately 130 kDa. The expected band size for ITGA2B is at 113 kDa.
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Immunohistochemical analysis of paraffin-embedded Human squamous carcinoma, using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human placenta, using the Antibody at 1:50 dilution.
Specific Publications For Anti-CD41 ITGA2B Rabbit Monoclonal Antibody (M01102)
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Customer Q&As
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4 Customer Q&As for Anti-CD41 ITGA2B Rabbit Monoclonal Antibody
Question
Is this M01102 anti-CD41 Rabbit Monoclonal antibody reactive to the isotypes of ITGA2B?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
The immunogen of M01102 anti-CD41 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD41. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-09
Question
I was wanting to use your anti-CD41 Rabbit Monoclonal antibody for WB for human fetal liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human fetal liver identification?
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
As indicated on the product datasheet, M01102 anti-CD41 Rabbit Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human fetal liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-06
Question
Can you help my question with product M01102, anti-CD41 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-11-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01102 anti-CD41 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-30
Question
I have attached the WB image, lot number and protocol we used for fetal liver using anti-CD41 Rabbit Monoclonal antibody M01102. Please let me know if you require anything else.
S. Kulkarni
Verified customer
Asked: 2013-07-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-07-23
