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- Table of Contents
CD44 antibody (A00052-2) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Flow Cytometry analysis of MCF-7 cells using anti-CD44 antibody (A00052-2).
Overlay histogram showing MCF-7 cells stained with A00052-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD44 Antibody (A00052-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing MCF-7 cells stained with A00052-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD44 Antibody (A00052-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-CD44 Antibody Picoband®
CD44 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
CD44 antibody was positively detected by WB in:
human Hela whole cell, human PANC-1 whole cell, human HeLa- WT whole cell, human HeLa-CD44 KO whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CD44 antibody was positively detected by IHC in:
human colon tissue, mouse lymphaden tissue, rat colon tissue, rat colon tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CD44 antibody was positively detected by ICC/IF in:
A431 cell
CD44 antibody was positively detected by FC in:
MCF-7 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-CD44 Antibody Picoband®
View all CD44 Antibodies
View all CD44 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human CD44 recombinant protein (Position: Q21-E518). Human CD44 shares 66.5% amino acid (aa) sequence identity with mouse CD44.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
85 kDa
Calculated Molecular Weight
81.5 kDa
Background of CD44
CD44 is also known as LHR or MC56. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Cite This Product
Anti-CD44 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00052-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
95%
High Homology
Dog
78%
Moderate Homology
Rabbit
76%
Moderate Homology
Mouse
71%
Moderate Homology
Rat
71%
Moderate Homology
Sheep
70%
Moderate Homology
Cow
68%
Low homology
Zebrafish
19%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CD44 antibody
Immunohistochemistry (IHC) protocol for CD44 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CD44 antibody
Flow Cytometry (FC) protocol for CD44 antibody
Publications
Application:
Species:
Genotype-dependent metabolic and inflammatory regulation at the ANRIL locus in human primary macrophages
Published: 2026-06-19
Journal: MOLECULAR GENETICS AND GENOMICS
Application: IF
Impact Factor: 2.20
Species: Human
PMID: 42319513
LncRNA WFDC21P mediates the tobacco carcinogen induced malignant transformation of human normal lung epithelial cells by regulating tumor stemness
Published: 2026-05-12
Journal: FOOD AND CHEMICAL TOXICOLOGY
Application: WB
Impact Factor: 3.20
Species: Human
PMID: 42128262
Genipin stabilized fibrin microbeads: Carrying cytokines to form niches for stem cell differentiation
Published: 2025-05-20
Journal: JOURNAL OF BIOMATERIALS APPLICATIONS
Application: IF
Impact Factor: 2.50
Species: Mouse
PMID: 40391599
MiR-21-5p Modulates Cisplatin-Resistance of CD44+ Gastric Cancer Stem Cells Through Regulating the TGF-β2/SMAD Signaling Pathway
Published: 2024-10-10
Journal: International Journal of General Medicine
Application: IHC
Impact Factor: 2.10
Species: Mouse
PMID: 39411053
Schwann cells promote neuronal differentiation of bone marrow stromal cells
Published: 2013-08-30
Journal: AFRICAN JOURNAL OF BIOTECHNOLOGY
Application: IF
Impact Factor: 0.00
Species: Rat
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Product Promise
We promise Anti-CD44 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A00052-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026