Anti-CD44 Antibody Picoband™

CD44 antibody

Boster Bio Anti-CD44 Antibody Picoband™ catalog # PB9333. Tested in IF, IHC, WB applications. This antibody reacts with Human, Rat. Cited in 37 publication(s). Independently reviewed in 2 review(s).

Product Info Summary

SKU: PB9333
Size: 100μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: IF, IHC, WB

Product Name

Anti-CD44 Antibody Picoband™

View all CD44 Antibodies

SKU/Catalog Number

PB9333

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD44 Antibody Picoband™ catalog # PB9333. Tested in IF, IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD44 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9333)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human CD44 (29-56aa RFAGVFHVEKNGRYSISRTEAADLCKAF), different from the related mouse and rat sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9333 is reactive to CD44 in Human, Rat

Applications

PB9333 is guaranteed for IF, IHC, WB Boster Guarantee

Background of CD44

CD44 is also known as LHR or MC56. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunofluorescence, 2μg/ml, Human, Rat

Validation Images & Assay Conditions

Gene/Protein Information For CD44 (Source: Uniprot.Org, NCBI)

Uniprot ID

P16070

Gene ID

960

Gene Name

CD44

Full Name

CD44 antigen

Weight

81.538kDa

Alternative Names

CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1 CD44 CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1 CD44 molecule (Indian blood group) CD44 antigen|GP90 lymphocyte homing/adhesion receptor|Hermes antigen|Indian blood group antigen|cell surface glycoprotein CD44|chondroitin sulfate proteoglycan 8|epican|extracellular matrix receptor III|hematopoietic cell E- and L-selectin ligand|heparan sulfate proteoglycan|homing function and Indian blood group system|hyaluronate receptor|phagocytic glycoprotein 1|soluble CD44

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD44, check out the CD44 Infographic

CD44 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD44: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9333 has been cited in 37 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Identification and differentiation therapy strategy of pterygium in vitro

Smad3-related miRNAs regulated oncogenic TRIB2 promoter activity to effectively suppress lung adenocarcinoma growth

Ternary Nanoparticles with a Sheddable Shell Efficiently Deliver MicroRNA-34a against CD44-Positive Melanoma

An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties

HER2 Interacts With CD44 to Up-regulate CXCR4 via Epigenetic Silencing of microRNA-139 in Gastric Cancer Cells
Species: Human

Kubatka P,Kello M,Kajo K,Samec M,Jasek K,Vybohova D,Uramova S,Liskova A,Sadlonova V,Koklesova L,Murin R,Adamkov M,Smejkal K,Svajdlenka E,Solar P,Samuel SM,Kassayova M,Kwon TK,Zubor P,Pec M,Danko J,Büsselberg D,Mojzis J.Chemopreventive and Therapeutic Efficacy of Cinnamomum zeylanicum L. Bark in Experimental Breast Carcinoma: Mechanistic In Vivo and In Vitro Analyses.Molecules.2020 Mar 19;25(6):1399.doi:10.3390/molecules25061399.PMID:32204409;PMCID:PMC7144360.
Species: Mouse,Rat
PB9333 usage in article: APP:IHC, SAMPLE:MAMMARY GLAND TISSUE, DILUTION:NA

Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
PB9333 usage in article: APP:WB, SAMPLE:MSC, DILUTION:NA

ACS Appl.Bio Mater.2020,XXXX,XXX,XXX-XXX Publication Date:December 15, 2020 https://doi.org/10.1021/acsabm.0c00927
Species: Human,Mouse
PB9333 usage in article: APP:IF, SAMPLE:4T1 CELL AND HUVECS, DILUTION:1:200

Zhang GF,Wu JC,Wang HY,Jiang WD,Qiu L. Overexpression of microRNA-205-5p exerts suppressive effects on stem cell drug resistance in gallbladder cancer by down-regulating PRKCE. Biosci Rep.2020 Sep 30;40(9): BSR20194509.doi:10.1042/BSR20194509.PMID: 328698
Species: Human,Mouse
PB9333 usage in article: APP:FLOW CYTOMETRY, SAMPLE:GBC STEM CELLS, DILUTION:NA

Kubatka P,Kello M,Kajo K,Samec M,Liskova A,Jasek K,Koklesova L,Kuruc T,Adamkov M,Smejkal K,Svajdlenka E,Solar P,Pec M,Büsselberg D,Sadlonova V,Mojzis J.Rhus coriaria L.(Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Bre
Species: Human,Mouse,Rat
PB9333 usage in article: APP:IHC, SAMPLE:MAMMARY TISSUE, DILUTION:NA

Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma

Rat nasal respiratory mucosa-derived ectomesenchymal stem cells differentiate into Schwann-like cells promoting the differentiation of PC12 cells and %u2026

Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.

Suspension culture combined with chemotherapeutic agents for sorting of breast cancer stem cells

Folium?Sennae?protects against hydroxyl radical-induced DNA damage via antioxidant mechanism: an?in vitro?study

Smad3-related miRNAs regulated oncogenic TRIB2 promoter activity to effectively suppress lung adenocarcinoma growth

Lentivirus-mediated PLC?1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma

Overexpression of transcription factor Foxa2 and Hnf1? induced rat bone mesenchymal stem cells into hepatocytes

Effects of BMSCs interactions with adventitial fibroblasts in transdifferentiation and ultrastructure processes

Fu W, Lv C, Zhuang W, Chen D, Lv E, Li F, Wang X. Neural Regen Res. 2013 Feb 15;8(5):427-34. Doi: 10.3969/J.Issn.1673-5374.2013.05.006. An Effective Inducer Of Dopaminergic Neuron-Like Differentiation.

Xue L, Liang H, Jiang X. Mol Med Rep. 2011 Nov-Dec;4(6):1233-7. Doi: 10.3892/Mmr.2011.552. Epub 2011 Aug 12. Up-Regulation Of Cd44 Expression By Interleukin-13 In A Murine Model Of Asthma.

Han M, Liu M, Wang Y, Mo Z, Bi X, Liu Z, Fan Y, Chen X, Wu C. Mol Cell Biochem. 2012 Apr;363(1-2):427-36. Doi: 10.1007/S11010-011-1195-5. Epub 2011 Dec 21. Re-Expression Of Mir-21 Contributes To Migration And Invasion By Inducing Epithelial-Mesenc...

Cui G, Qi Z, Guo X, Qin J, Gui Y, Cai Z. Cell Biol Int. 2009 Mar;33(3):434-41. Rat Bone Marrow Derived Mesenchymal Progenitor Cells Support Mouse Es Cell Growth And Germ-Like Cell Differentiation.

Hefei W, Yu R, Haiqing W, Xiao W, Jingyuan W, Dongjun L. Cell Biol Int. 2015 Mar;39(3):253-63. Doi: 10.1002/Cbin.10387. Epub 2015 Jan 26. Morphological Characteristics And Identification Of Islet-Like Cells Derived From Rat Adipose-Derived Stem Ce...

Hou Y, Zou Q, Ge R, Shen F, Wang Y. Cell Res. 2012 Jan;22(1):259-72. Doi: 10.1038/Cr.2011.139. Epub 2011 Aug 23. The Critical Role Of Cd133(+)Cd44(+/High) Tumor Cells In Hematogenous Metastasis Of Liver Cancers.

Shi S, Han L, Deng L, Zhang Y, Shen H, Gong T, Zhang Z, Sun X. J Control Release. 2014 Nov 28;194:228-37. Doi: 10.1016/J.Jconrel.2014.09.005. Epub 2014 Sep 16. Dual Drugs (Microrna-34A And Paclitaxel)-Loaded Functional Solid Lipid Nanoparticles Fo...

Huang Y, Chang T, Yang C, Wu M. J Huazhong Univ Sci Technolog Med Sci. 2012 Aug;32(4):620-5. Doi: 10.1007/S11596-012-1007-5. Epub 2012 Aug 11. Mineralized And Osteoid Tissue From Dental Pulp Stem Cells On Micro-Arc Oxidation Titanium In Vitro.

Han M, Liu M, Wang Y, Chen X, Xu J, Sun Y, Zhao L, Qu H, Fan Y, Wu C. Plos One. 2012;7(6):E39520. Doi: 10.1371/Journal.Pone.0039520. Epub 2012 Jun 25. Antagonism Of Mir-21 Reverses Epithelial-Mesenchymal Transition And Cancer Stem Cell Phenotype T...

Chen Df, Zhang Hl, Du Sh, Li H, Zhou Jh, Li Yw, Zeng Hp, Hua Zc. Steroids. 2010 Dec 12;75(13-14):1119-26. Doi: 10.1016/J.Steroids.2010.07.009. Epub 2010 Jul 29. Cholesterol Myristate Suppresses The Apoptosis Of Mesenchymal Stem Cells Via Upregulat...

Dai K, Chen R, Ding Y, Niu Z, Fan J, Xu C. Cells Tissues Organs. 2014;200(2):132-40. Doi: 10.1159/000380762. Epub 2015 Apr 16. Induction Of Functional Hepatocyte-Like Cells By Overexpression Of Foxa3 And Hnf4?? In Rat Bone Marrow Mesenchymal Stem ...

Li X, Huang Y, Zheng L, Liu H, Niu X, Huang J, Zhao F, Fan Y. J Biomed Mater Res A. 2014 Apr;102(4):1092-101. Doi: 10.1002/Jbm.A.34774. Epub 2013 Jun 11. Effect Of Substrate Stiffness On The Functions Of Rat Bone Marrow And Adipose Tissue Derived ...

Dai J, Li Y, Zhou H, Chen J, Chen M, Xiao Z. Int J Biol Sci. 2013 Nov 21;9(10):1089-98. Doi: 10.7150/Ijbs.7367. Ecollection 2013. Genistein Promotion Of Osteogenic Differentiation Through Bmp2/Smad5/Runx2 Signaling.

Li Hm, Liu L, Mei X, Chen H, Liu Z, Zhao X. Life Sci. 2014 Jun 13;106(1-2):50-7. Doi: 10.1016/J.Lfs.2014.04.020. Epub 2014 Apr 26. Overexpression Of Inducible Nitric Oxide Synthase Impairs The Survival Of Bone Marrow Stem Cells Transplanted Into R...

Zhang G, Lan X, Yen Tc, Chen Q, Pei Z, Qin C, Zhang Y. Nucl Med Biol. 2012 Nov;39(8):1243-50. Doi: 10.1016/J.Nucmedbio.2012.06.010. Epub 2012 Jul 15. Therapeutic Gene Expression In Transduced Mesenchymal Stem Cells Can Be Monitored Using A Reporte...

Huang Xf, Yuan Sj, Yang C. Mol Med Rep. 2012 Sep;6(3):547-52. Doi: 10.3892/Mmr.2012.974. Epub 2012 Jun 29. Effects Of Total Flavonoids From Drynaria??fortunei On The Proliferation And Osteogenic Differentiation Of Rat Dental Pulp Stem ...

Du G, Liu Y, Dang M, Zhu G, Su R, Fan Y, Tan Z, Wang Lx, Fang J. Acta Histochem. 2014 Jul;116(6):1075-84. Doi: 10.1016/J.Acthis.2014.05.002. Epub 2014 Jun 21. Comparison Of Administration Routes For Adipose-Derived Stem Cells In The Treatment Of M...

Li B, Chu X, Gao M, Li W. Acta Biochim Biophys Sin (Shanghai). 2010 Jan;42(1):80-9. Apoptotic Mechanism Of Mcf-7 Breast Cells In Vivo And In Vitro Induced By Photodynamic Therapy With C-Phycocyanin.

Have you used Anti-CD44 Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

2 Reviews For Anti-CD44 Antibody Picoband™

0

Good enough for publishing--Chun-Chih Tseng, NORRIS CANCER CENTER, UNIVERSITY OF SOUTHERN CALIFORNIA, RESEARCH ASSISTANT

Excellent

Source: Biocompare.com

Applications Western Blot
Sample Human breast cancer cell line
Detection Bio-Rad imager

"I used this anti-CD44 C-terminus antibody to detect cleaved/non-cleaved CD44 product. The results for Western blot are okay. The signal (1:1500) is not super strong but good enough to see clear bands. This antibody could detect a band the same size as another antibody that I used against the extracellular region of CD44, indicating this antibody could detect CD44. CD44 has multiple spliced isoforms. The WB result showed multiple bands, some of the bands I am interested in overlapped with the bands generated by another CD44 antibody targeting the extracellular domains. So, the specificity of this antibody is probably okay. The intensity of bands are acceptable, good enough for publishing. Relatively inexpnsive, okay specificity. Good enough to detect CD44 C-term."

Verified customer

Submitted

0

High specificity and stability--Elena Deryugina, CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR

Excellent

Source: Biocompare.com

Applications Western Blot
Sample Tumor cell lysate and recombinant protein
Detection SuperSignal West Pico Chemiluminescent Substrate

"The rabbit antibody PB9333 from Boster produced very good results in western blotting with the lysate of human CD44-positive cancer cells as well as with the recombinant N-terminal protein. The detection was specific, resulting only in the protein bands of expected mol. weights. The antibody is also capable of detecting shed CD44 in conditioned medium. High specificity and stability. I would highly recommend this antibody for detection of CD44 in human cells by western blotting."

Verified customer

Submitted

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

16 Customer Q&As for Anti-CD44 Antibody Picoband™

Question

I am looking for using your anti-CD44 antibody for positive regulation of peptidyl-serine phosphorylation studies. Has this antibody been tested with western blotting on intestinal cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

We appreciate your inquiry. This PB9333 anti-CD44 antibody is validated on hela whole cell lysate, panc whole cell lysate, intestinal cancer tissue. It is guaranteed to work for IF, IHC-P, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-31

Question

I am interested in to test anti-CD44 antibody PB9333 on human keratinocyte for research purposes, then I may be interested in using anti-CD44 antibody PB9333 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

The products we sell, including anti-CD44 antibody PB9333, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-04

Question

Is this PB9333 anti-CD44 antibody reactive to the isotypes of CD44?

Verified Customer

Verified customer

Asked: 2019-12-18

Answer

The immunogen of PB9333 anti-CD44 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human CD44(29-56aa RFAGVFHVEKNGRYSISRTEAADLCKAF), different from the related mouse and rat sequences by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-18

Question

We purchased anti-CD44 antibody for IF on reticulocyte in a previous project. I am using rat, and I plan to use the antibody for IHC-P next. We need examining reticulocyte as well as glial tumor in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

I took a look at the website and datasheets of our anti-CD44 antibody and it appears that PB9333 has been tested on rat in both IF and IHC-P. Thus PB9333 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-10-15

Question

Would anti-CD44 antibody PB9333 work for IF with keratinocyte?

Verified Customer

Verified customer

Asked: 2019-09-02

Answer

According to the expression profile of keratinocyte, CD44 is highly expressed in keratinocyte. So, it is likely that anti-CD44 antibody PB9333 will work for IF with keratinocyte.

Boster Scientific Support

Answered: 2019-09-02

Question

Is a blocking peptide available for product anti-CD44 antibody (PB9333)?

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

We do provide the blocking peptide for product anti-CD44 antibody (PB9333). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-07

Question

I was wanting to use your anti-CD44 antibody for IF for human keratinocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human keratinocyte identification?

Verified Customer

Verified customer

Asked: 2019-07-12

Answer

As indicated on the product datasheet, PB9333 anti-CD44 antibody has been tested for IF, IHC-P, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human keratinocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-12

Question

I see that the anti-CD44 antibody PB9333 works with IF, what is the protocol used to produce the result images on the product page?

M. Collins

Verified customer

Asked: 2018-10-31

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-10-31

Question

We have seen staining in rat articular cartilage. Any tips? Is anti-CD44 antibody supposed to stain articular cartilage positively?

Verified Customer

Verified customer

Asked: 2018-06-25

Answer

Based on literature articular cartilage does express CD44. Based on Uniprot.org, CD44 is expressed in parotid gland, reticulocyte, myeloid leukemia cell, keratinocyte, lymphoblast, mammary carcinoma, articular cartilage, colon adenocarcinoma retinal pigment epithelium, spinal cord, pancreas retinal pigment epithelium, glial tumor, peripheral blood, foreskin, lung, plasma, cervix carcinoma, t-cell, liver, leukemic t-cell, among other tissues. Regarding which tissues have CD44 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231, 23186163
Foreskin, Pubmed ID: 2007624
Glial tumor, Pubmed ID: 7527301
Keratinocyte, Pubmed ID: 1281868
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 1717145, 7528188
Lymphoblast, Pubmed ID: 1465456, 1922057
Mammary carcinoma, Pubmed ID: 8352881
Myeloid leukemia cell, Pubmed ID: 2056274
Pancreas, and Retinal pigment epithelium, Pubmed ID: 15489334
Peripheral blood, Pubmed ID: 7508992
Plasma, Pubmed ID: 16335952
Reticulocyte, Pubmed ID: 1840487
Spinal cord, Pubmed ID: 17974005
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2018-06-25

Question

We are currently using anti-CD44 antibody PB9333 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-04-19

Answer

The anti-CD44 antibody (PB9333) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-04-19

Question

Our lab were content with the WB result of your anti-CD44 antibody. However we have observed positive staining in spinal cord cell membrane using this antibody. Is that expected? Could you tell me where is CD44 supposed to be expressed?

T. Miller

Verified customer

Asked: 2017-02-06

Answer

According to literature, spinal cord does express CD44. Generally CD44 expresses in cell membrane. Regarding which tissues have CD44 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231, 23186163
Foreskin, Pubmed ID: 2007624
Glial tumor, Pubmed ID: 7527301
Keratinocyte, Pubmed ID: 1281868
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 1717145, 7528188
Lymphoblast, Pubmed ID: 1465456, 1922057
Mammary carcinoma, Pubmed ID: 8352881
Myeloid leukemia cell, Pubmed ID: 2056274
Pancreas, and Retinal pigment epithelium, Pubmed ID: 15489334
Peripheral blood, Pubmed ID: 7508992
Plasma, Pubmed ID: 16335952
Reticulocyte, Pubmed ID: 1840487
Spinal cord, Pubmed ID: 17974005
T-cell, Pubmed ID: 19367720

Boster Scientific Support

Answered: 2017-02-06

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for keratinocyte using anti-CD44 antibody PB9333. Let me know if you need anything else.

D. Wu

Verified customer

Asked: 2016-09-12

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-09-12

Question

I have a question about product PB9333, anti-CD44 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

P. Krishna

Verified customer

Asked: 2015-08-31

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9333 anti-CD44 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-08-31

Question

Here is the WB image, lot number and protocol we used for keratinocyte using anti-CD44 antibody PB9333. Please let me know if you require anything else.

J. Singh

Verified customer

Asked: 2015-04-14

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-04-14

Question

Do you have a BSA free version of anti-CD44 antibody PB9333 available?

C. Wu

Verified customer

Asked: 2014-12-24

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD44 antibody PB9333 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-12-24

Question

Will PB9333 anti-CD44 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

V. Kulkarni

Verified customer

Asked: 2013-12-17

Answer

As indicated on the product datasheet, PB9333 anti-CD44 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-12-17

Size

Total: $315

SKU:PB9333

Ask a question

Get A Quote

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

Product Categories

Primary Antibodies

Primary and Secondary Antibodies

Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. Buy a primary antibody and get a secondary antibody for free!

ELISA Kits

ELISA Kits

More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. We offer our Boster-branded Picokine™ ELISA kits (sandwich ELISA) and EZ-Set™ ELISA kits (DIY antibody pairs) in addition to many multiplex ELISA kits.

Image

Recombinant Proteins

Boster provides a wide range of human, mouse, and rat recombinant proteins, which include cytokines, growth factors, chemokines, and many more. Our recombinant proteins have high stability, biological activity, and purity.

Boster Kit

Reagents

Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, and MTT assay kits.

In stock
Order Product
PB9333
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.