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- Table of Contents
CD44 antibody (PB9333) performs well in IF, IHC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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IHC analysis of CD44 using anti-CD44 antibody (PB9333).
CD44 was detected in a paraffin-embedded section of Human Intestinal Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD44 Antibody (PB9333) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
CD44 was detected in a paraffin-embedded section of Human Intestinal Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD44 Antibody (PB9333) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-CD44 Antibody Picoband®
CD44 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | IF, IHC, WB |
Tested Applications
CD44 antibody was positively detected by WB in:
human Hela whole cell, human U87 whole cell, rat PC-12 whole cell, rat C6 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CD44 antibody was positively detected by IHC in:
Human Intestinal Cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CD44 antibody was positively detected by IF in:
human tonsil tissue, rat lymphaden tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunofluorescence
2μg/ml
Human, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Rat
Tested
Recommend
Tested
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-CD44 Antibody Picoband®
View all CD44 Antibodies
View all CD44 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CD44, different from the related mouse and rat sequences by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
82 kDa
Calculated Molecular Weight
81.5 kDa
Background of CD44
CD44 is also known as LHR or MC56. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Cite This Product
Anti-CD44 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9333)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
96%
High Homology
Dog
96%
High Homology
Mouse
93%
High Homology
Rat
93%
High Homology
Cow
93%
High Homology
Sheep
93%
High Homology
Zebrafish
39%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CD44 antibody
Immunohistochemistry (IHC) protocol for CD44 antibody
Immunofluorescence (IF) protocol for CD44 antibody
Publications
Application:
Species:
An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties
Published: 2021-11-19
Journal: BIOMATERIALS
Application: IF
Impact Factor: 12.48
Species: Rat
PMID: 34823885
Antioxidant and Cytoprotective effects of Pyrola decorata H. Andres and its five phenolic components
Published: 2019-10-21
Journal: BMC Complementary and Alternative Medicine
Application: FC
Impact Factor: 2.48
Species: Rat
PMID: 31638966
Anticancer Activities of Thymus vulgaris L. in Experimental Breast Carcinoma In Vivo and In Vitro
Published: 2019-04-09
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: IHC
Impact Factor: 4.18
Species: Rat
PMID: 30970626
Identification and differentiation therapy strategy of pterygium in vitro
Published: 2018-08-15
Journal: American Journal of Translational Research
Application: IHC,IF
Impact Factor: 3.06
Species: Mouse
PMID: 30210698
Ternary Nanoparticles with a Sheddable Shell Efficiently Deliver MicroRNA-34a against CD44-Positive Melanoma
Published: 2017-08-14
Journal: MOLECULAR PHARMACEUTICS
Application: IHC
Impact Factor: 4.44
Species: Mouse
PMID: 28759238
Smad3-related miRNAs regulated oncogenic TRIB2 promoter activity to effectively suppress lung adenocarcinoma growth
Published: 2016-12-22
Journal: Cell Death & Disease
Application: IHC
Impact Factor: 5.38
Species: Mouse
PMID: 28005074
Dual drugs (microRNA-34a and paclitaxel)-loaded functional solid lipid nanoparticles for synergistic cancer cell suppression
Published: 2014-09-16
Journal: JOURNAL OF CONTROLLED RELEASE
Application: IHC
Impact Factor: 7.26
Species: Mouse
PMID: 25220161
Review
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2 Reviews For Anti-CD44 Antibody Picoband®
Good enough for publishing--Chun-Chih Tseng, NORRIS CANCER CENTER, UNIVERSITY OF SOUTHERN CALIFORNIA, RESEARCH ASSISTANT
Source: Biocompare.com
| SKU | PB9333 |
|---|---|
| Applications | Western Blot |
| Sample | Human breast cancer cell line |
| Detection | Bio-Rad imager |
"I used this anti-CD44 C-terminus antibody to detect cleaved/non-cleaved CD44 product. The results for Western blot are okay. The signal (1:1500) is not super strong but good enough to see clear bands. This antibody could detect a band the same size as another antibody that I used against the extracellular region of CD44, indicating this antibody could detect CD44. CD44 has multiple spliced isoforms. The WB result showed multiple bands, some of the bands I am interested in overlapped with the bands generated by another CD44 antibody targeting the extracellular domains. So, the specificity of this antibody is probably okay. The intensity of bands are acceptable, good enough for publishing. Relatively inexpnsive, okay specificity. Good enough to detect CD44 C-term."
Chun-Chih Tseng
Verified customer
Submitted 2016-08-24
High specificity and stability--Elena Deryugina, CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR
Source: Biocompare.com
| SKU | PB9333 |
|---|---|
| Applications | Western Blot |
| Sample | Tumor cell lysate and recombinant protein |
| Detection | SuperSignal West Pico Chemiluminescent Substrate |
"The rabbit antibody PB9333 from Boster produced very good results in western blotting with the lysate of human CD44-positive cancer cells as well as with the recombinant N-terminal protein. The detection was specific, resulting only in the protein bands of expected mol. weights. The antibody is also capable of detecting shed CD44 in conditioned medium. High specificity and stability. I would highly recommend this antibody for detection of CD44 in human cells by western blotting."
Elena Deryugina
Verified customer
Submitted 2016-08-24
Product Promise
We promise Anti-CD44 Antibody Picoband® performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9333
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026