Product Info Summary
SKU: | A00555-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-CD45/PTPRC Antibody Picoband™
SKU/Catalog Number
A00555-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD45/PTPRC Antibody Picoband™ catalog # A00555-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD45/PTPRC Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00555-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD45/PTPRC recombinant protein (Position: Q26-S185).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00555-2 is reactive to PTPRC in Human, Mouse
Applications
A00555-2 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
200 kDa
Calculated molecular weight
147.486kDa
Background of PTPRC
CD45 (Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene.CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase. The cytogenetic location of CD45 is 1q31.3-q32.1. CD45 is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD45 using anti-CD45 antibody (A00555-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: mouse thymus tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD45 antigen affinity purified polyclonal antibody (Catalog # A00555-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD45 at approximately 200KD. The expected band size for CD45 is at 147KD.
Protein Target Info & Infographic
Gene/Protein Information For PTPRC (Source: Uniprot.org, NCBI)
Gene Name
PTPRC
Full Name
Receptor-type tyrosine-protein phosphatase C
Weight
147.486kDa
Superfamily
protein-tyrosine phosphatase family
Alternative Names
B220; CD45 antigen; CD45; CD45R; EC 3.1.3.48; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide PTPRC B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200 protein tyrosine phosphatase receptor type C receptor-type tyrosine-protein phosphatase C|CD45 antigen|T200 glycoprotein|T200 leukocyte common antigen|protein tyrosine phosphatase, receptor type, c polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTPRC, check out the PTPRC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTPRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD45/PTPRC Antibody Picoband™ (A00555-2)
Hello CJ!
A00555-2 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of human umbilical cord mesenchymal stem cells on endometriotic cell proliferation and apoptosis
Lipoxin A4 and its analogue suppress the tumor growth of transplanted H22 in mice: the role of antiangiogenesis
Detecting cell-in-cell structures in human tumor samples by E-cadherin/CD68/CD45 triple staining
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-CD45/PTPRC Antibody Picoband™
Question
We were well pleased with the WB result of your anti-CD45/PTPRC antibody. However we have observed positive staining in leukemic t-cell cell membrane using this antibody. Is that expected? Could you tell me where is PTPRC supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
From what I have seen in literature, leukemic t-cell does express PTPRC. Generally PTPRC expresses in cell membrane. Regarding which tissues have PTPRC expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218
Lymphocyte, Pubmed ID: 2824653
Placenta, Pubmed ID: 2971730, 2845400
Synovium, Pubmed ID: 14702039
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2020-04-21
Question
I am interested in using your anti-CD45/PTPRC antibody for severe combined immunodeficiency autosomal recessive t- studies. Has this antibody been tested with western blotting on mouse thymus tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
Thank you for your inquiry. This A00555-2 anti-CD45/PTPRC antibody is tested on mouse thymus tissue. It is guaranteed to work for ELISA, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-17
Question
We have seen staining in human t-cell. Do you have any suggestions? Is anti-CD45/PTPRC antibody supposed to stain t-cell positively?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
From literature t-cell does express PTPRC. From Uniprot.org, PTPRC is expressed in blood, lymphocyte, synovium, placenta, t-cell, liver, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have PTPRC expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218
Lymphocyte, Pubmed ID: 2824653
Placenta, Pubmed ID: 2971730, 2845400
Synovium, Pubmed ID: 14702039
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2020-01-07
Question
I see that the anti-CD45/PTPRC antibody A00555-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-18
Question
I am interested in to test anti-CD45/PTPRC antibody A00555-2 on mouse t-cell for research purposes, then I may be interested in using anti-CD45/PTPRC antibody A00555-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The products we sell, including anti-CD45/PTPRC antibody A00555-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-08
Question
Would anti-CD45/PTPRC antibody A00555-2 work for WB with t-cell?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
According to the expression profile of t-cell, PTPRC is highly expressed in t-cell. So, it is likely that anti-CD45/PTPRC antibody A00555-2 will work for WB with t-cell.
Boster Scientific Support
Answered: 2019-09-12
Question
Would A00555-2 anti-CD45/PTPRC antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
You can see on the product datasheet, A00555-2 anti-CD45/PTPRC antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-23
Question
Can you help my question with product A00555-2, anti-CD45/PTPRC antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00555-2 anti-CD45/PTPRC antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-17
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-CD45/PTPRC antibody A00555-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-30
Question
Do you have a BSA free version of anti-CD45/PTPRC antibody A00555-2 available?
B. Collins
Verified customer
Asked: 2018-04-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD45/PTPRC antibody A00555-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-11
Question
We purchased anti-CD45/PTPRC antibody for WB on liver last year. I am using human, and We want to use the antibody for ELISA next. My lab would like examining liver as well as erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
M. Carter
Verified customer
Asked: 2017-09-14
Answer
I viewed the website and datasheets of our anti-CD45/PTPRC antibody and it appears that A00555-2 has been validated on human in both WB and ELISA. Thus A00555-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-09-14
Question
Is a blocking peptide available for product anti-CD45/PTPRC antibody (A00555-2)?
J. Edwards
Verified customer
Asked: 2017-09-13
Answer
We do provide the blocking peptide for product anti-CD45/PTPRC antibody (A00555-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-13
Question
We are currently using anti-CD45/PTPRC antibody A00555-2 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
The anti-CD45/PTPRC antibody (A00555-2) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-28
Question
Is this A00555-2 anti-CD45/PTPRC antibody reactive to the isotypes of PTPRC?
B. Mitchell
Verified customer
Asked: 2016-10-11
Answer
The immunogen of A00555-2 anti-CD45/PTPRC antibody is E.coli-derived human CD45/PTPRC recombinant protein (Position: Q26-S185). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-10-11
Question
Please see the WB image, lot number and protocol we used for t-cell using anti-CD45/PTPRC antibody A00555-2. Please let me know if you require anything else.
D. Rodriguez
Verified customer
Asked: 2016-08-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-08-25
Question
I was wanting to use your anti-CD45/PTPRC antibody for WB for mouse t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse t-cell identification?
P. Kulkarni
Verified customer
Asked: 2014-03-25
Answer
It shows on the product datasheet, A00555-2 anti-CD45/PTPRC antibody has been tested for ELISA, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-03-25