Anti-CD5 Antibody Picoband™

Boster Bio Anti-CD5 Antibody Picoband™ catalog # A00480-2. Tested in ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Independently reviewed in 1 review(s).

Product Info Summary

SKU: A00480-2
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB

Product Name

Anti-CD5 Antibody Picoband™

See all CD5 products

SKU/Catalog Number

A00480-2

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD5 Antibody Picoband™ catalog # A00480-2. Tested in ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00480-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human CD5 recombinant protein (Position: R25-L495).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00480-2 is reactive to CD5 in Human, Mouse, Rat

Applications

A00480-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For CD5 (Source: Uniprot.Org, NCBI)

Uniprot ID

P06127

Gene ID

921

Gene Name

CD5

Full Name

T-cell surface glycoprotein CD5

Weight

54.578kDa

Alternative Names

CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1 CD5 LEU1, T1 CD5 molecule T-cell surface glycoprotein CD5|CD5 antigen (p56-62)|epididymis secretory sperm binding protein|lymphocyte antigen T1/Leu-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD5, check out the CD5 Infographic

CD5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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1 Reviews For Anti-CD5 Antibody Picoband™

0

Immunohistochemistry for Anti-CD5 Antibody

Excellent

Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Tonsil
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 0.5 ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min

Verified customer

Submitted

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16 Customer Q&As for Anti-CD5 Antibody Picoband™

Question

We were well pleased with the WB result of your anti-CD5 antibody. However we have observed positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is CD5 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-05-04

Answer

According to literature, leukocyte does express CD5. Generally CD5 expresses in cell membrane. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-05-04

Question

Would A00480-2 anti-CD5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

N. Carter

Verified customer

Asked: 2020-03-27

Answer

You can see on the product datasheet, A00480-2 anti-CD5 antibody as been validated on IHC-F. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-27

Question

I have a question about product A00480-2, anti-CD5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-30

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00480-2 anti-CD5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-30

Question

Does anti-CD5 antibody A00480-2 work for IHC-F with thymus?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

According to the expression profile of thymus, CD5 is highly expressed in thymus. So, it is likely that anti-CD5 antibody A00480-2 will work for IHC-F with thymus.

Boster Scientific Support

Answered: 2019-09-25

Question

Is a blocking peptide available for product anti-CD5 antibody (A00480-2)?

Verified Customer

Verified customer

Asked: 2019-09-10

Answer

We do provide the blocking peptide for product anti-CD5 antibody (A00480-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-10

Question

I see that the anti-CD5 antibody A00480-2 works with IHC-F, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

You can find protocols for IHC-F on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-16

Question

I am interested in to test anti-CD5 antibody A00480-2 on mouse thymus for research purposes, then I may be interested in using anti-CD5 antibody A00480-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

The products we sell, including anti-CD5 antibody A00480-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-06-03

Question

We want using your anti-CD5 antibody for cell recognition studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-05-14

Answer

I appreciate your inquiry. This A00480-2 anti-CD5 antibody is validated on human tonsil tissue, jurkat whole cell lysates, mouse spleen tissue, nih3t3 whole cell lysates. It is guaranteed to work for ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-05-14

Question

I was wanting to use your anti-CD5 antibody for IHC-F for mouse thymus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse thymus identification?

Verified Customer

Verified customer

Asked: 2018-09-11

Answer

As indicated on the product datasheet, A00480-2 anti-CD5 antibody has been validated for ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse thymus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-09-11

Question

See below the WB image, lot number and protocol we used for thymus using anti-CD5 antibody A00480-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-08-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-08-21

Question

We have tried in the past anti-CD5 antibody for IF on thymus in the past. I am using rat, and We intend to use the antibody for ELISA next. I am looking for examining thymus as well as lymphocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

F. Dhar

Verified customer

Asked: 2018-07-30

Answer

I looked at the website and datasheets of our anti-CD5 antibody and it seems that A00480-2 has been validated on rat in both IF and ELISA. Thus A00480-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-07-30

Question

We have seen staining in human leukocyte. What should we do? Is anti-CD5 antibody supposed to stain leukocyte positively?

Verified Customer

Verified customer

Asked: 2018-07-30

Answer

From what I have seen in literature leukocyte does express CD5. From what I have seen in Uniprot.org, CD5 is expressed in leukocyte, lymphocyte, thymus, pancreas, leukemic t-cell, among other tissues. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-07-30

Question

We are currently using anti-CD5 antibody A00480-2 for human tissue, and we are content with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2018-06-22

Answer

The anti-CD5 antibody (A00480-2) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-06-22

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-CD5 antibody A00480-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-01-17

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-17

Question

Is this A00480-2 anti-CD5 antibody reactive to the isotypes of CD5?

C. Baker

Verified customer

Asked: 2015-03-19

Answer

The immunogen of A00480-2 anti-CD5 antibody is E. coli-derived human CD5 recombinant protein (Position: R25-L495). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2015-03-19

Question

Is there a BSA free version of anti-CD5 antibody A00480-2 available?

G. Gonzalez

Verified customer

Asked: 2013-05-13

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD5 antibody A00480-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-05-13

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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A00480-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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