Anti-CD5 Antibody Picoband™ (monoclonal, 4E2)

Boster Bio Anti-CD5 Antibody Picoband™ (monoclonal, 4E2) catalog # M00480-2. Tested in IHC-P, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M00480-2
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: IHC-P, WB

Product Name

Anti-CD5 Antibody Picoband™ (monoclonal, 4E2)

See all CD5 products

SKU/Catalog Number

M00480-2

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD5 Antibody Picoband™ (monoclonal, 4E2) catalog # M00480-2. Tested in IHC-P, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD5 Antibody Picoband™ (monoclonal, 4E2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00480-2)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

4E2

Isotype

IgG1

Immunogen

E. coli-derived human CD5 recombinant protein (Position: R25-L495).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M00480-2 is reactive to CD5 in Human

Applications

M00480-2 is guaranteed for IHC-P, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat

Validation Images & Assay Conditions

Gene/Protein Information For CD5 (Source: Uniprot.Org, NCBI)

Uniprot ID

P06127

Gene ID

921

Gene Name

CD5

Full Name

T-cell surface glycoprotein CD5

Weight

54.578kDa

Alternative Names

CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1 CD5 LEU1, T1 CD5 molecule T-cell surface glycoprotein CD5|CD5 antigen (p56-62)|epididymis secretory sperm binding protein|lymphocyte antigen T1/Leu-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD5, check out the CD5 Infographic

CD5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Anti-CD5 Antibody Picoband™ (monoclonal, 4E2)

Question

We are currently using anti-CD5 antibody (monoclonal, 4E2) M00480-2 for human tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

The anti-CD5 antibody (monoclonal, 4E2) (M00480-2) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-22

Question

We bought anti-CD5 antibody (monoclonal, 4E2) for IHC-P on thymus a few months ago. I am using human, and We intend to use the antibody for WB next. I am interested in examining thymus as well as leukemic t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

I have checked the website and datasheets of our anti-CD5 antibody (monoclonal, 4E2) and it appears that M00480-2 has been tested on human in both IHC-P and WB. Thus M00480-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-01-10

Question

Our lab were well pleased with the WB result of your anti-CD5 antibody (monoclonal, 4E2). However we have seen positive staining in pancreas cell membrane using this antibody. Is that expected? Could you tell me where is CD5 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-07-02

Answer

From literature, pancreas does express CD5. Generally CD5 expresses in cell membrane. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-07-02

Question

We have observed staining in human thymus. Any tips? Is anti-CD5 antibody (monoclonal, 4E2) supposed to stain thymus positively?

R. Krishna

Verified customer

Asked: 2013-05-06

Answer

From what I have seen in literature thymus does express CD5. From what I have seen in Uniprot.org, CD5 is expressed in leukocyte, lymphocyte, thymus, pancreas, leukemic t-cell, among other tissues. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2013-05-06

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M00480-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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