Click image to see more details
Product Info Summary
| SKU: | M06249 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD53 Monoclonal Antibody
SKU/Catalog Number
M06249
BM5750 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD53 Monoclonal Antibody catalog # M06249. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD53 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06249)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFFG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD53 May be involved in growth regulation in hematopoietic cells.
Reactive Species
M06249 is reactive to CD53 in Human
Observed Molecular Weight
24 kDa
Calculated molecular weight
24.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06249 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of CD53 expression in Jurkat cell lysate.
Specific Publications For Anti-CD53 Monoclonal Antibody (M06249)
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4 Customer Q&As for Anti-CD53 Monoclonal Antibody
Question
Why the expected band size of 24 kDa can't be seen. Instead there is a band showing up at approx. 35 kDa. What is the cause?
Verified customer
Asked: 2022-01-26
Answer
M06249 is a membrane protein, and it will have post-translational modifications that cause the actual molecular weight to be larger than theoretical MW.
Boster Scientific Support
Answered: 2022-01-27
Question
I see that the anti-CD53 Monoclonal antibody M06249 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-04
Question
Is a blocking peptide available for product anti-CD53 Monoclonal antibody (M06249)?
Verified Customer
Verified customer
Asked: 2018-09-07
Answer
We do provide the blocking peptide for product anti-CD53 Monoclonal antibody (M06249). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-09-07
Question
We are currently using anti-CD53 Monoclonal antibody M06249 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
C. Gonzalez
Verified customer
Asked: 2016-06-30
Answer
The anti-CD53 Monoclonal antibody (M06249) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-06-30
