Product Info Summary
SKU: | A00910-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, ICC, WB |
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Product info
Product Name
Anti-CD55 Antibody
SKU/Catalog Number
A00910-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CD55 Antibody catalog # A00910-2. Tested in WB,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD55 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00910-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human DDR1 around the phosphorylation site of Y513.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00910-2 is reactive to CD55 in Human, Mouse, Rat
Applications
A00910-2 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
41.4kDa
Background of CD55/DAF
Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'.
Gotoh I., J. Biol. Chem. 276:4276-4286(2001).
Bloem L.J., J. Mol. Cell. Cardiol. 33:1739-1750(2001).
Gross E.A., J. Biol. Chem. 277:13873-13882(2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunofluorescent staining data of CD55 using Anti-CD55 Antibody (A00910-2).
Immunofluorescence analysis of A549 cellsClick image to see more details
Figure 1. Western blotting validation for Anti-CD55 Antibody A00910-2
WesternBlot (WB) analysis of CD55 polyclonal antibody in extracts from Hela and A549 cells.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CD55 (Source: Uniprot.org, NCBI)
Gene Name
CD55
Full Name
Complement decay-accelerating factor
Weight
41.4kDa
Superfamily
receptors of complement activation (RCA) family
Alternative Names
CD55 antigen; CD55 molecule, decay accelerating factor for complement (Cromer blood group); CD55; CR; CRdecay accelerating factor for complement (CD55, Cromer blood group system); CROMDAFcomplement decay-accelerating factor; DAF; decay accelerating factor for complement; TC CD55 CHAPLE, CR, CROM, DAF, TC CD55 molecule (Cromer blood group) complement decay-accelerating factor|CD55 antigen|CD55 molecule, decay accelerating factor for complement (Cromer blood group)|Cromer blood group antigen|Rh blood group D antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD55, check out the CD55 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD55: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD55 Antibody (A00910-2)
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No publications found for A00910-2
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