Anti-CD59 Rabbit Monoclonal Antibody

CD59 antibody

Boster Bio Anti-CD59 Rabbit Monoclonal Antibody catalog # M00914-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M00914-1
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IP, IF, ICC, WB

Product Name

Anti-CD59 Rabbit Monoclonal Antibody

View all CD59 Antibodies

SKU/Catalog Number

M00914-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CD59 Rabbit Monoclonal Antibody catalog # M00914-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD59 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00914-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AABE-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CD59

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00914-1 is reactive to CD59 in Human

Applications

M00914-1 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

120 kDa

Calculated molecular weight

14.177kDa

Background of CD59

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CD59 (Source: Uniprot.org, NCBI)

Gene Name

CD59

Full Name

CD59 glycoprotein

Weight

14.177kDa

Alternative Names

16.3A5; 1F5 antigen; 1F5; 20 kDa homologous restriction factor; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16; CD59 antigen; CD59 antigen, complement regulatory protein; CD59 glycoprotein; CD59 molecule, complement regulatory protein; CD59; EJ16; EJ30; EJ30, EL32 and G344); EL32; FLJ38134; FLJ92039; G344; HRF20; HRF-20; human leukocyte antigen MIC11; Ly-6-like protein; lymphocytic antigen CD59/MEM43; MACIF; MAC-inhibitory protein; MAC-IP; MEM43 antigen; MEM43; membrane attack complex (MAC) inhibition factor; Membrane attack complex inhibition factor; Membrane in CD59 16.3A5, 1F5, EJ16, EJ30, EL32, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20 CD59 molecule (CD59 blood group) CD59 glycoprotein|1F5 antigen|20 kDa homologous restriction factor|CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344)|CD59 blood group antigen|CD59 molecule, complement regulatory protein|Ly-6-like protein|MEM43 antigen|T cell-activating protein|human leukocyte antigen MIC11|lymphocytic antigen CD59/MEM43|membrane attack complex (MAC) inhibition factor|membrane attack complex inhibition factor|membrane inhibitor of reactive lysis|protectin|surface anitgen recognized by monoclonal antibody 16.3A5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD59, check out the CD59 Infographic

CD59 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD59: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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15 Customer Q&As for Anti-CD59 Rabbit Monoclonal Antibody

Question

Is this M00914-1 anti-CD59 Rabbit Monoclonal antibody reactive to the isotypes of CD59?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

The immunogen of M00914-1 anti-CD59 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD59. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-27

Question

Will M00914-1 anti-CD59 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-01

Answer

You can see on the product datasheet, M00914-1 anti-CD59 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-01

Question

Is a blocking peptide available for product anti-CD59 Rabbit Monoclonal antibody (M00914-1)?

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

We do provide the blocking peptide for product anti-CD59 Rabbit Monoclonal antibody (M00914-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-18

Question

Our lab want to know about to test anti-CD59 Rabbit Monoclonal antibody M00914-1 on human blood for research purposes, then I may be interested in using anti-CD59 Rabbit Monoclonal antibody M00914-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

The products we sell, including anti-CD59 Rabbit Monoclonal antibody M00914-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-19

Question

We purchased anti-CD59 Rabbit Monoclonal antibody for IP on milk in a previous experiment. I am using human, and We are going to use the antibody for WB next. you antibody examining milk as well as colon in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

I have checked the website and datasheets of our anti-CD59 Rabbit Monoclonal antibody and it seems that M00914-1 has been tested on human in both IP and WB. Thus M00914-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-07-08

Question

We are currently using anti-CD59 Rabbit Monoclonal antibody M00914-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-11-02

Answer

The anti-CD59 Rabbit Monoclonal antibody (M00914-1) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-11-02

Question

I was wanting to use your anti-CD59 Rabbit Monoclonal antibody for IF for human blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human blood identification?

Verified Customer

Verified customer

Asked: 2018-08-16

Answer

It shows on the product datasheet, M00914-1 anti-CD59 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-08-16

Question

Our team were satisfied with the WB result of your anti-CD59 Rabbit Monoclonal antibody. However we have been able to see positive staining in chorionic villus cell membrane using this antibody. Is that expected? Could you tell me where is CD59 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-12-27

Answer

According to literature, chorionic villus does express CD59. Generally CD59 expresses in cell membrane. Regarding which tissues have CD59 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1383553
Colon, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Milk, Pubmed ID: 18780401
T-cell, Pubmed ID: 2475570
Urine, Pubmed ID: 7520819

Boster Scientific Support

Answered: 2017-12-27

Question

We have observed staining in human urine. Are there any suggestions? Is anti-CD59 Rabbit Monoclonal antibody supposed to stain urine positively?

Verified Customer

Verified customer

Asked: 2017-10-19

Answer

According to literature urine does express CD59. According to Uniprot.org, CD59 is expressed in chorionic villus, t-cell, blood, colon, milk, liver, urine, among other tissues. Regarding which tissues have CD59 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 1383553
Colon, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Milk, Pubmed ID: 18780401
T-cell, Pubmed ID: 2475570
Urine, Pubmed ID: 7520819

Boster Scientific Support

Answered: 2017-10-19

Question

Do you have a BSA free version of anti-CD59 Rabbit Monoclonal antibody M00914-1 available?

K. Moore

Verified customer

Asked: 2017-10-05

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD59 Rabbit Monoclonal antibody M00914-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-10-05

Question

Please see the WB image, lot number and protocol we used for blood using anti-CD59 Rabbit Monoclonal antibody M00914-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-09-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-09-28

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-CD59 Rabbit Monoclonal antibody M00914-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-07-14

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-14

Question

I see that the anti-CD59 Rabbit Monoclonal antibody M00914-1 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-07-13

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-07-13

Question

Will anti-CD59 Rabbit Monoclonal antibody M00914-1 work for IF with blood?

H. Brown

Verified customer

Asked: 2016-08-05

Answer

According to the expression profile of blood, CD59 is highly expressed in blood. So, it is likely that anti-CD59 Rabbit Monoclonal antibody M00914-1 will work for IF with blood.

Boster Scientific Support

Answered: 2016-08-05

Question

I have a question about product M00914-1, anti-CD59 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Parker

Verified customer

Asked: 2014-11-05

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00914-1 anti-CD59 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-11-05

Order DetailsPrice
M00914-1

100μL

$370
M00914-1-30ul

30μL sample

$99
M00914-1-carrier-free

Carrier Free

$370

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Free Secondary Antibody

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M00914-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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