Rabbit IgG polyclonal antibody for P-selectin(SELP) detection. Tested with WB, IHC-P in Human. Cited in 1 publication(s).
Anti-CD62P/SELP Antibody Info At A Glance
|Product Name||Anti-CD62P/SELP Antibody
See all P-Selectin/CD62P primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for P SELECTIN/SELP detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. P SELECTIN/SELP information: Molecular Weight: 90834 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein; Tissue Specificity: Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.|
|Cite This Product||Anti-CD62P/SELP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1715)|
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|Anti-CD62P/SELP Antibody may replace the following items: sc 101336|sc 101619|sc 18834|sc 19672|sc 19996|sc 22637|sc 22639|sc 25771|sc 271267|sc 30162|sc 376858|sc 59769|sc 6941|sc 6943|sc 8419.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human CD62P(49-66aa KAYSWNISRKYCQNRYTD).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For SELP (Source: Uniprot.org, NCBI)
|NCBI Gene Id||6403|
|Species Of This Entry||Human|
|Alternative Names||P-Selectin/CD62P|CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP|
|Post Tranlational Modifications||Phosphorylation; Nitration; Cleavage; Oxidation|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein.|
|Gene Location||1q24.2, on Chromasome 1, gene sequence: NC_000001.11 (169588849..169630125, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For SELP (Source: Uniprot.org, NCBI)
|Protein Function||Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG.|
|Tissue Specificity||Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.|
|Related Diseases||Tissue Adhesions; Inflammation; Malignant Neoplasms; Atherosclerosis; Cardiovascular Diseases; Neoplasms; Heart Diseases; Inflammatory Response; Malignant Head And Neck Neoplasm; Von Willebrand Disease; Infarction; Myocardial Infarction; Head And Neck Neoplasms; Malignant Neck Neoplasm; Coronary Artery Disease; Thrombosis; Dysequilibrium Syndrome; Coronary Heart Disease; Diabetes Mellitus; Neoplasm Metastasis|
|Related Pathways||Cell Adhesion; Platelet Activation; Pathogenesis; Inflammatory Response; Platelet Aggregation; Aging; Cell Activation; Immune Response; Endothelial Cell Activation; Angiogenesis; Transport; Hypersensitivity; Virulence; Spermatogenesis; Fermentation; Translation; Cell Cycle; Fibrinolysis; Innate Immune Response; Cognition|
|Background||P-Selectin, also called CD62, GRMP, GMP140 is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. The P-Selectin gene is mapped on 1q24.2. P-selectin plays an essential role in the initial recruitment of leukocytes(white blood cells) to the site of injury during inflammation. P-selectin is also very important in the recruitment and aggregation of platelets at areas of vascular injury. In quiescent platelet, P-selectin is located on the inner wall of alpha-granules. Sorting nexin-17 interacts with the cytosolic domain of P-selectin and suggested that SNX17 may function in the intracellular trafficking of P-selectin. P-selectin expression was found to be significantly and positively correlated with carotid artery stiffness and wall thickness, and the percentage of P-selectin-positive platelets was significantly higher in patients with carotid plaque.|
|Scientific References||PMID: 2466574 by Johnston G.I., et al. Cloning of GMP-140, a granule membrane protein of platelets and endothelium: sequence similarity to proteins involved in cell adhesion and inflammation.|
PMID: 7684381 by Fujimoto T., et al. P-selectin is acylated with palmitic acid and stearic acid at cysteine 766 through a thioester linkage.
Our Boster Quality Guarantee for Anti-CD62P/SELP Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti CD62P (PA1715) at 0.5ug/ml
WB: PANC Whole Cell Lysate at 40ug
Predicted bind size: 91KD
Observed bind size: 140KD
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Placenta Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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