Click image to see more details
Product Info Summary
| SKU: | M01241 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD62P SELP Rabbit Monoclonal Antibody
SKU/Catalog Number
M01241
BM5032 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD62P SELP Rabbit Monoclonal Antibody catalog # M01241. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD62P SELP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01241)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAEC-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD62P
Reactive Species
M01241 is reactive to SELP in Human
Observed Molecular Weight
91 kDa
Calculated molecular weight
90.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01241 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: human placenta tissue, human HEL whole cell, human THP-1 whole cell, human SH-SY5Y whole cell, human K562 whole cell, human Jurkat whole cell, human SIHA whole cell, human RT4 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of P-Selectin/SELP using anti-P-Selectin/SELP antibody (M01241).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human Jurkat whole cell lysates,
Lane 7: human SIHA whole cell lysates,
Lane 8: human RT4 whole cell lysates,
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-Selectin/SELP antigen affinity purified monoclonal antibody (M01241) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P-Selectin/SELP at approximately 91 kDa. The expected band size for P-Selectin/SELP is at 91 kDa.
Specific Publications For Anti-CD62P SELP Rabbit Monoclonal Antibody (M01241)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD62P SELP Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-CD62P SELP Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-CD62P SELP Rabbit Monoclonal Antibody
Question
Will anti-CD62P Rabbit Monoclonal antibody M01241 work for WB with platelet?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
According to the expression profile of platelet, SELP is highly expressed in platelet. So, it is likely that anti-CD62P Rabbit Monoclonal antibody M01241 will work for WB with platelet.
Boster Scientific Support
Answered: 2020-03-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-CD62P Rabbit Monoclonal antibody M01241. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-03
Question
My question regarding product M01241, anti-CD62P Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01241 anti-CD62P Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-25
Question
Would anti-CD62P Rabbit Monoclonal antibody M01241 work on primate WB with adrenal gland?
C. Walker
Verified customer
Asked: 2016-06-06
Answer
Our lab technicians have not validated anti-CD62P Rabbit Monoclonal antibody M01241 on primate. You can run a BLAST between primate and the immunogen sequence of anti-CD62P Rabbit Monoclonal antibody M01241 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate adrenal gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-06-06
Question
Does M01241 anti-CD62P Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Edwards
Verified customer
Asked: 2015-08-17
Answer
It shows on the product datasheet, M01241 anti-CD62P Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-08-17
