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Product Info Summary
| SKU: | A02428-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-CD64/FCGR1A Antibody Picoband®
SKU/Catalog Number
A02428-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD64/FCGR1A Antibody Picoband® catalog # A02428-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CD64/FCGR1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02428-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD64/FCGR1A recombinant protein (Position: A191-D254).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02428-3 is reactive to FCGR1A in Human, Mouse, Rat
Observed Molecular Weight
70 kDa
Calculated molecular weight
42.6 kDa
Background of FCGR1A
High affinity immunoglobulin gamma Fc receptor I is a protein that in humans is encoded by the FCGR1A gene. It is mapped to 1q21.2. This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02428-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human THP-1 whole cell, rat spleen tissue, rat thymus tissue, rat PC-12 whole cell, mouse EL-4 whole cell, mouse spleen tissue, mouse thymus tissue, mouse RAW2647 whole cell, mouse ANA-1 whole cell
FCM: SiHa cell
Validation Images & Assay Conditions
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Western blot analysis of CD64/FCGR1A using anti-CD64/FCGR1A antibody (A02428-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse EL-4 whole cell lysates,
Lane 6: mouse spleen tissue lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse RAW264.7 whole cell lysates,
Lane 9: mouse ANA-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD64/FCGR1A antigen affinity purified polyclonal antibody (Catalog # A02428-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD64/FCGR1A at approximately 70 kDa. The expected band size for CD64/FCGR1A is at 43 kDa.
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Flow Cytometry analysis of SiHa cells using anti-CD64/FCGR1A antibody (A02428-3).
Overlay histogram showing SiHa cells stained with A02428-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD64/FCGR1A Antibody (A02428-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-CD64/FCGR1A Antibody Picoband® (A02428-3)
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