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- Table of Contents
CD80 antibody (M00196-1) performs well in ELISA, Flow Cytometry, IF, IHC-P, ICC and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow cytometry analysis of CD80 overexpressing HEK293 cells using CD80 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: CD80 overexpressing HEK293 cells.
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Anti-CD80 Monoclonal Antibody [8G12]
CD80 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | ELISA, Flow Cytometry, IF, IHC-P, ICC |
Recommend Dilution
Application
Dilution
Species
CD80 antibody can be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 5 μg/mL. Flow cytometry at 10 μg/ml.
Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-CD80 Monoclonal Antibody [8G12]
View all CD80 Antibodies
View all CD80 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
CD80 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Mouse
Contents
CD80 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Clonality
Monoclonal
Clone Number
Clone: 8G12
Isotype
IgG2b
Immunogen
CD80 antibody was raised against the extracellular domain of human CD80.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
33.0 kDa
Background of CD80
CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
Cite This Product
Anti-CD80 Monoclonal Antibody [8G12] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00196-1)
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Product Promise
We promise Anti-CD80 Monoclonal Antibody [8G12] performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC-P, ICC. If it doesn't perform as promised, we will refund the product. Learn more
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M00196-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026