Anti-Cd80 Antibody Picoband®

B7-1/CD80 antibody

Boster Bio Anti-Cd80 Antibody Picoband® catalog # A00196-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A00196-3
Size: 100 μg/vial
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, Flow Cytometry, WB

Product Name

Anti-Cd80 Antibody Picoband®

View all B7-1/CD80 Antibodies

SKU/Catalog Number

A00196-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cd80 Antibody Picoband® catalog # A00196-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Cd80 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00196-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Cd80 recombinant protein (Position: D62-K92).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00196-3 is reactive to Cd80 in Human, Mouse

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

70 kDa

Calculated molecular weight

33.048kDa

Background of B7-1/CD80

Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00196-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/ml, -

Positive Control

WB: human Daudi whole cell, human K562 whole cell, mouse RAW2647 whole cell, mouse ANA-1 whole cell
FCM: ANA-1 cell

Validation Images & Assay Conditions

Gene/Protein Information For Cd80 (Source: Uniprot.org, NCBI)

Gene Name

Cd80

Full Name

T-lymphocyte activation antigen CD80

Weight

33.048kDa

Alternative Names

T-lymphocyte activation antigen CD80; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7; CD80; CD80; CD28LG; CD28LG1; LAB7 CD80 B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7 CD80 molecule T-lymphocyte activation CD80|B-lymphocyte activation B7|CD80 (CD28 ligand 1, B7-1 )|CTLA-4 counter-receptor B7.1|activation B7-1 |costimulatory factor CD80|costimulatory molecule variant IgV-CD80

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Cd80, check out the Cd80 Infographic

Cd80 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Cd80: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A00196-3

100μg

$370
A00196-3-10ug

10μg sample (liquid)

$99
A00196-3-Biotin

100 μg Biotin conjugated

$570
A00196-3-Cy3

100 μg Cy3 conjugated

$570
A00196-3-Dylight488

100 μg Dylight488 conjugated

$570
A00196-3-Dylight550

100 μg Dylight550 conjugated

$570
A00196-3-Dylight594

100 μg Dylight594 conjugated

$570
A00196-3-FITC

100 μg FITC conjugated

$570
A00196-3-HRP

100 μg HRP conjugated

$570
A00196-3-APC

100 μg APC conjugated

$670
A00196-3-PE

100 μg PE conjugated

$670
A00196-3-iFluor647

100 μg iFluor647 conjugated

$670
A00196-3-carrier-free

Carrier Free

$370

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A00196-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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