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Product Info Summary
| SKU: | M00196 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD80/B7 1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00196
BM4933 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD80/B7 1 Rabbit Monoclonal Antibody catalog # M00196. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD80/B7 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00196)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOEH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD80
Reactive Species
M00196 is reactive to CD80 in Human
Observed Molecular Weight
60 kDa
Calculated molecular weight
33.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00196 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of CD80 expression in Raji cell lysate.
Specific Publications For Anti-CD80/B7 1 Rabbit Monoclonal Antibody (M00196)
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Customer Q&As
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5 Customer Q&As for Anti-CD80/B7 1 Rabbit Monoclonal Antibody
Question
We have observed staining in human brain. Any tips? Is anti-CD80/B7 1 Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
According to literature brain does express CD80. According to Uniprot.org, CD80 is expressed in gastrocnemius, lymphoid tissue, brain, erythroleukemia, among other tissues. Regarding which tissues have CD80 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Lymphoid tissue, Pubmed ID: 2794510, 1377173
Boster Scientific Support
Answered: 2020-04-30
Question
I was wanting to use using your anti-CD80/B7 1 Rabbit Monoclonal antibody for positive regulation of t-helper 1 cell differentiation studies. Has this antibody been tested with western blotting on raji cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
Thanks for your inquiry. This M00196 anti-CD80/B7 1 Rabbit Monoclonal antibody is tested on raji cell lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-24
Question
We purchased anti-CD80/B7 1 Rabbit Monoclonal antibody for WB on gastrocnemius in the past. I am using human, and We intend to use the antibody for IHC next. I am looking for examining gastrocnemius as well as erythroleukemia in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
I looked at the website and datasheets of our anti-CD80/B7 1 Rabbit Monoclonal antibody and I see that M00196 has been tested on human in both WB and IHC. Thus M00196 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-26
Question
My boss were satisfied with the WB result of your anti-CD80/B7 1 Rabbit Monoclonal antibody. However we have observed positive staining in lymphoid tissue membrane using this antibody. Is that expected? Could you tell me where is CD80 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-10-26
Answer
According to literature, lymphoid tissue does express CD80. Generally CD80 expresses in membrane. Regarding which tissues have CD80 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Lymphoid tissue, Pubmed ID: 2794510, 1377173
Boster Scientific Support
Answered: 2018-10-26
Question
We are currently using anti-CD80/B7 1 Rabbit Monoclonal antibody M00196 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-28
Answer
The anti-CD80/B7 1 Rabbit Monoclonal antibody (M00196) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-28
