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Cd86 antibody (A00220-4) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 21 Publications

a00220 4 cd86 primary antibodies fcm testing 3_1

Flow Cytometry analysis of ANA-1 cells using anti-Cd86 antibody (A00220-4).
Overlay histogram showing ANA-1 cells stained with A00220-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Cd86 Antibody (A00220-4, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-Cd86 Antibody Picoband®

Name: Anti-Cd86 Antibody Picoband®
Brand: Boster Bio
SKU: A00220-4
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

Cd86 antibody

21 Publications|

1 Review

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Reactive Species	Host	Application
Mouse, Rat	Rabbit	ELISA, Flow Cytometry, IHC, WB

Tested Applications

Cd86 antibody was positively detected by WB in:

rat lung tissue, mouse lung tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Cd86 antibody was positively detected by IHC in:

rat spleen tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Cd86 antibody was positively detected by FC in:

ANA-1 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Rat

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-Cd86 Antibody Picoband®
View all CD86 Antibodies

Form

Lyophilized

Uniprot

P42082

Gene ID

12524

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Cd86 recombinant protein (Position: T35-E221). Mouse Cd86 shares 61.9 % amino acid (aa) sequence identity with human CD86.

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

60-80 kDa

Calculated Molecular Weight

34.7 kDa

Background of Cd86

Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

Cite This Product

Anti-Cd86 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00220-4)

Protocols

Western Blot protocol for Cd86 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for Cd86 antibody

Review Protocol

Flow Cytometry (FC) protocol for Cd86 antibody

Review Protocol

Publications

Formononetin attenuates cerebral ischemia-reperfusion injury by regulating microglial glycolysis through AMPK

Published: 2026-03-24

Journal: JOURNAL OF ETHNOPHARMACOLOGY

Application: WB

Impact Factor: 5.40

Species: Mouse

PMID: 41887382

SKU	A00220-4
Application	Immunofluorescence
Sample	Normal and preterm human placenta
Sample Processing Description	Clinically collected term and preterm placentas were processed for paraffin embedding and sectioning in the longitudinal orientation, allowing visualization of both the amnion and chorion.
Other Reagents	Goat serum, DAPI (AR1176,Boster Bio),Anti-fade mounting medium
Primary Antibody	Cd86 Antibody Picoband®
Primary Incubation	1:200, overnight at 4 ℃
Secondary Antibody	DyLight 594 Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:500, 1 hour in room temperature
Detection	Imaging system:Leica DM2500
Results Summary	CD86 is a marker of M1 macrophages. In normal placenta, macrophages are generally absent or very few, and M1 macrophages are even less. In preterm placenta, due to inflammation, the number of macrophages increases and they tend to polarize toward the M1 type. Our experimental results strongly support this theory and are consistent with the expected data.

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Anti-Cd86 Antibody Picoband®

Cd86 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Formononetin attenuates cerebral ischemia-reperfusion injury by regulating microglial glycolysis through AMPK

Mechanisms of IL-17A Neutralisation in Alleviating Renal Fibrosis and Inflammation in Spontaneously Hypertensive Rats

Photobiomodulation mitigates chondrocyte catabolism in osteoarthritis by modulating macrophage M1 polarization through the IL-6/JAK/STAT pathway

TNIP3 Attenuates Colitis by Inhibiting the TAB1/NF-κB/NLRP3 Signaling Pathway in Macrophages

In Situ Programming of the Tumor Microenvironment to Alleviate Immunosuppression for Pancreatic Cancer Immunotherapy

Poliumoside alleviates microglia-mediated inflammation and blood-brain barrier disruption via modulating the polarization of microglia after ischemic stroke in mice

Calycosin-7-glucoside-Loaded Hydrogel Promotes Wound Healing in Gestational Diabetes Mellitus

Alleviation of Microglia Mediating Hippocampal Neuron Impairments and Depression-Related Behaviors by Urolithin B via the SIRT1-FOXO1 Pathway

Yunvjian decoction attenuates lipopolysaccharide-induced acute lung injury by inhibiting NF-κB/NLRP3 pathway and pyroptosis

Porcine reproductive and respiratory syndrome virus infects the reproductive system of male piglets and impairs development of the blood–testis barrier

Oral microsphere formulation of M2 macrophage-mimetic Janus nanomotor for targeted therapy of ulcerative colitis

AMPK Regulates M1 Macrophage Polarization through the JAK2/STAT3 Signaling Pathway to Attenuate Airway Inflammation in Obesity-Related Asthma

“Butterfly-effect” nano-platforms for cascade immune amplification and tumor clearance through multi-modal therapy and immune sensitization

Dynamic processes of positive/negative immune-vascular crosstalk loop via functional MRI-guided monitor

Cu+/Ca2+ Dual-Overload Strategy Assisted by Metabolic-Symbiotic-Destruction for Tumor Apoptosis and Tumor Immune Activation

Triptolide attenuates LPS-induced activation of RAW 264.7 macrophages by inducing M1-to-M2 repolarization via the mTOR/STAT3 signaling

Surface modification of titanium implants by pH-Responsive coating designed for Self-Adaptive antibacterial and promoted osseointegration

Near infrared light-triggered on-demand Cur release from Gel-PDA@Cur composite hydrogel for antibacterial wound healing

Lithium chloride promoted hematoma resolution after intracerebral hemorrhage through GSK-3β-mediated pathways-dependent microglia phagocytosis and M2-phenotype differentiation, angiogenesis and neurogenesis in a rat model

Copper-nanoparticle-embedded hydrogel for killing bacteria and promoting wound healing with photothermal therapy

Macrophage polarization and activation at the interface of multi-walled carbon nanotube-induced pulmonary inflammation and fibrosis

Review

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1 Review For Anti-Cd86 Antibody Picoband®

The antibody has high specificity and strong potency, producing excellent results.

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