Anti-CDC34/Ubch3 Rabbit Monoclonal Antibody

Product Name

Anti-CDC34/Ubch3 Rabbit Monoclonal Antibody

View all UBE2R1/CDC34 Antibodies

SKU/Catalog Number

M03038

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CDC34/Ubch3 Rabbit Monoclonal Antibody catalog # M03038. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CDC34/Ubch3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03038)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

IBO-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CDC34

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03038 is reactive to CDC34 in Human, Mouse, Rat

Applications

M03038 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

56 kDa

Calculated molecular weight

26.737kDa

Background of UBE2R1/CDC34

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

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Western blot analysis of CDC34 expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.

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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

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Immunofluorescent analysis of Hela cells, using CDC34 Antibody.

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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Gene/Protein Information For CDC34 (Source: Uniprot.org, NCBI)

Alternative Names

Cdc34; cell division cycle 34 homolog (S. cerevisiae); cell division cycle 34; E2-CDC34; EC 6.3.2.19; UBC3; UBE2R1; UBE2R1UBCH3; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1 CDC34 E2-CDC34, UBC3, UBCH3, UBE2R1 cell division cycle 34, ubiqiutin conjugating enzyme ubiquitin-conjugating enzyme E2 R1|(E3-independent) E2 ubiquitin-conjugating enzyme R1|E2 ubiquitin-conjugating enzyme R1|cell division cycle 34 homolog|ubiquitin carrier protein|ubiquitin-conjugating enzyme E2-32 KDA complementing|ubiquitin-conjugating enzyme E2-CDC34|ubiquitin-protein ligase R1

For more info on CDC34, check out the CDC34 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CDC34: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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