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Product Info Summary
| SKU: | A30515 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MOL1A MOB1B Antibody
SKU/Catalog Number
A30515
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MOL1A MOB1B Antibody (Catalog# A30515). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MOL1A MOB1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30515)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human MOL1A. Immunogen sequence location: 101-150
Reactive Species
A30515 is reactive to MOB1B in Human, Monkey, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
25.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30515 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of various cells using Mob1A Polyclonal Antibody
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Western blot analysis of lysates from 293, COS7, and HUVEC cells, treated with IFN 2500U/ml 30', using MOL1A Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-MOL1A MOB1B Antibody (A30515)
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Customer Q&As
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7 Customer Q&As for Anti-MOL1A MOB1B Antibody
Question
I was wanting to use your anti-MOL1A antibody for WB for mouse renal medulla on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse renal medulla identification?
B. Edwards
Verified customer
Asked: 2020-01-24
Answer
It shows on the product datasheet, A30515 anti-MOL1A antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse renal medulla in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-24
Question
I see that the anti-MOL1A antibody A30515 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-29
Question
Will A30515 anti-MOL1A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
As indicated on the product datasheet, A30515 anti-MOL1A antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-15
Question
Do you have a BSA free version of anti-MOL1A antibody A30515 available?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MOL1A antibody A30515 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-22
Question
We are currently using anti-MOL1A antibody A30515 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
The anti-MOL1A antibody (A30515) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-11
Question
Will anti-MOL1A antibody A30515 work for WB with renal medulla?
K. Roberts
Verified customer
Asked: 2015-06-22
Answer
According to the expression profile of renal medulla, MOB1B is highly expressed in renal medulla. So, it is likely that anti-MOL1A antibody A30515 will work for WB with renal medulla.
Boster Scientific Support
Answered: 2015-06-22
Question
Can you help my question with product A30515, anti-MOL1A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Wu
Verified customer
Asked: 2013-02-26
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30515 anti-MOL1A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-02-26
