Product Info Summary
SKU: | A02169 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Cdc37 (H9) Antibody
SKU/Catalog Number
A02169
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cdc37 (H9) Antibody catalog # A02169. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cdc37 (H9) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02169)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Rad17 around the phosphorylation site of S646.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02169 is reactive to CDC37 in Human, Mouse, Rat
Applications
A02169 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
44.468kDa
Background of CDC37
Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity. Deconjugates SUMO1 from CLOCK, which decreases its transcriptional activation activity.
Taihei Yamaguchi, Mol. Cell. Biol., Jun 2005; 25: 5171 - 5182.
Jinke Cheng, Mol. Cell. Biol., Jul 2004; 24: 6021 - 6028.
Molly A. Brewer, AACR Meeting Abstracts, Mar 2004; 2004: 1006.
Imke M. Veltman, Hum. Mol. Genet., Jul 2005; 14: 1955 - 1963.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of CDC37 using Anti-Cdc37 (H9) Antibody (A02169).
Immunohistochemistry (IHC) analyzes of Cdc37 (H9) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-Cdc37 (H9) Antibody A02169
Western blot (WB) analysis of Cdc37 (H9) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug)Lane2:Hela whole cell lysate(40ug)
Lane3:C6 whole cell lysate(40ug)
Lane4:MEF whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CDC37 (Source: Uniprot.org, NCBI)
Gene Name
CDC37
Full Name
Hsp90 co-chaperone Cdc37
Weight
44.468kDa
Superfamily
CDC37 family
Alternative Names
CDC37 (cell division cycle 37, S. cerevisiae, homolog); CDC37 cell division cycle 37 homolog (S. cerevisiae); CDC37 cell division cycle 37 homolog; CDC37A; cell division cycle 37 homolog (S. cerevisiae); Hsp90 chaperone protein kinase-targeting subunit; hsp90 co-chaperone Cdc37; P50CDC37 CDC37 P50CDC37 cell division cycle 37, HSP90 cochaperone hsp90 co-chaperone Cdc37|CDC37 (cell division cycle 37, S. cerevisiae, homolog)|CDC37 cell division cycle 37 homolog|cell division cycle 37 homolog|hsp90 chaperone protein kinase-targeting subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC37, check out the CDC37 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC37: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cdc37 (H9) Antibody (A02169)
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No publications found for A02169
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Customer Reviews
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