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Product Info Summary
| SKU: | M01367 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CDC45/Cdc45L Rabbit Monoclonal Antibody
SKU/Catalog Number
M01367
BM4772 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CDC45/Cdc45L Rabbit Monoclonal Antibody catalog # M01367. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDC45/Cdc45L Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01367)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CDC45
Reactive Species
M01367 is reactive to CDC45 in Human, Mouse, Rat
Observed Molecular Weight
65 kDa
Calculated molecular weight
65.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01367 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of CDC45 expression in Jurkat cell lysate.
Specific Publications For Anti-CDC45/Cdc45L Rabbit Monoclonal Antibody (M01367)
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Customer Q&As
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4 Customer Q&As for Anti-CDC45/Cdc45L Rabbit Monoclonal Antibody
Question
I see that the anti-CDC45/Cdc45L Rabbit Monoclonal antibody M01367 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-06-18
Question
I was wanting to use your anti-CDC45/Cdc45L Rabbit Monoclonal antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2018-07-25
Answer
It shows on the product datasheet, M01367 anti-CDC45/Cdc45L Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-25
Question
My lab would like to test anti-CDC45/Cdc45L Rabbit Monoclonal antibody M01367 on rat cervix carcinoma for research purposes, then I may be interested in using anti-CDC45/Cdc45L Rabbit Monoclonal antibody M01367 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-09-11
Answer
The products we sell, including anti-CDC45/Cdc45L Rabbit Monoclonal antibody M01367, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-11
Question
My question regarding product M01367, anti-CDC45/Cdc45L Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Patel
Verified customer
Asked: 2016-12-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01367 anti-CDC45/Cdc45L Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-12-08
