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Product Info Summary
| SKU: | PA2123 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CDC5L Antibody Picoband®
SKU/Catalog Number
PA2123
BA3109-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CDC5L Antibody catalog # PA2123. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDC5L Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2123)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CDC5L, different from the related rat and mouse sequences by four amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2123 is reactive to CDC5L in Human, Rat
Observed Molecular Weight
100 kDa
Calculated molecular weight
92.3 kDa
Background of CDC5L
Cell division cycle 5-like protein, also called PCDC5RP or CDC5-like, is a protein that in humans is encoded by the CDC5L gene. The CDC5L gene was mapped to chromosome 6p21 by fluorescence in situ hybridization. This DNA-binding protein involved in cell cycle control. It may act as a transcription activator. The Component of the PRP19-CDC5L complex forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The role of CDC5 (Polo-like kinase) in regulating Rho1 is likely to be relevant to cytokinesis and asymmetric cell division in other organisms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2123 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: human HEK293 whole cell, human THP-1 whole cell, rat C6 whole cell,
Validation Images & Assay Conditions
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Western blot analysis of CDC5L using anti-CDC5L antibody (PA2123).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates
Lane 2: human THP-1 whole cell lysates
Lane 3: rat C6 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC5L antigen affinity purified polyclonal antibody (Catalog # PA2123) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC5L at approximately 100KD. The expected band size for CDC5L is at 92KD.
Specific Publications For Anti-CDC5L Antibody Picoband® (PA2123)
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1 Customer Q&As for Anti-CDC5L Antibody Picoband®
Question
We are currently using anti-CDC5L antibody PA2123 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on pig tissues as well?
D. Lewis
Verified customer
Asked: 2013-05-08
Answer
The anti-CDC5L antibody (PA2123) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-05-08
