Product Info Summary
SKU: | A07293 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-CDCA2 Antibody
SKU/Catalog Number
A07293
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CDCA2 Antibody (Catalog # A07293). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDCA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07293)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human CDCA2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07293 is reactive to Cdca2 in Human
Applications
A07293 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
106.366kDa
Background of Cdca2
Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates.
Walker M.G., Curr. Cancer Drug Targets 1:73-83(2001).
Ota T., . Genet. 36:40-45(2004).
Olsen J.V., Cell 127:635-648(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from 293 cells, using CDCA2 antibody A07293.
Protein Target Info & Infographic
Gene/Protein Information For Cdca2 (Source: Uniprot.org, NCBI)
Gene Name
Cdca2
Full Name
Cell division cycle-associated protein 2
Weight
106.366kDa
Alternative Names
cell division cycle associated 2; cell division cycle-associated protein 2; FLJ25804; MGC129906; MGC129907; protein phosphatase 1 [gamma] (PPP1R3A) binding protein Repo-Man; Repo-ManRecruits PP1 onto mitotic chromatin at anaphase protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cdca2, check out the Cdca2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cdca2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDCA2 Antibody (A07293)
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No publications found for A07293
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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5 Customer Q&As for Anti-CDCA2 Antibody
Question
Does anti-CDCA2 antibody A07293 work on bovine WB with skin testis?
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
Our lab technicians have not validated anti-CDCA2 antibody A07293 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CDCA2 antibody A07293 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine skin testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-15
Question
We are currently using anti-CDCA2 antibody A07293 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
The anti-CDCA2 antibody (A07293) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-20
Question
Will A07293 anti-CDCA2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
It shows on the product datasheet, A07293 anti-CDCA2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-03
Question
I have a question about product A07293, anti-CDCA2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07293 anti-CDCA2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-25
Question
Will anti-CDCA2 antibody A07293 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
According to the expression profile of cervix carcinoma erythroleukemia, CDCA2 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-CDCA2 antibody A07293 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-09-03