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Product Info Summary
| SKU: | A13390-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-CDH20 Antibody (N-term)
View all Cadherin-20 Antibodies
SKU/Catalog Number
A13390-2
Size
80 µl
Description
Boster Bio Anti-CDH20 Antibody (N-term) (Catalog # A13390-2). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CDH20 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13390-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CDH20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the N-terminal region of human CDH20.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13390-2 is reactive to CDH20 in Human
Reconstitution
Calculated molecular weight
88993 Da
Background of Cadherin-20
This gene is a type II classical cadherin from the cadherin superfamily and one of three cadherin 7-like genes located in a cluster on chromosome 18. The encoded membrane protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Since disturbance of intracellular adhesion is a prerequisite for invasion and metastasis of tumor cells, cadherins are considered prime candidates for tumor suppressor genes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13390-2 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of lysates from human brain and mouse brain tissue lysate (from left to right), using CDH20 Antibody (N-term). A13390-2 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Western blot analysis of CDH20 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). CDH20 (arrow) was detected using the purified Pab.
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CDH20 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CDH20 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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CDH20 Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For CDH20 (Source: Uniprot.org, NCBI)
Gene Name
CDH20
Full Name
Cadherin-20
Weight
88993 Da
Alternative Names
Cadherin-20, CDH20, CDH7L3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDH20, check out the CDH20 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDH20: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDH20 Antibody (N-term) (A13390-2)
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