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CDK2 antibody (M00166-3) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Flow Cytometry analysis of ANA-1 cells using anti-Cdk2 antibody (M00166-3).
Overlay histogram showing ANA-1 cells stained with M00166-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cdk2 Antibody (M00166-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing ANA-1 cells stained with M00166-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cdk2 Antibody (M00166-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Cdk2 Antibody Picoband® (monoclonal, 5B12D1)
CDK2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | Flow Cytometry, IHC, WB |
Tested Applications
CDK2 antibody was positively detected by WB in:
human Jurkat whole cell, human HepG2 whole cell, human K562 whole cell, rat heart tissue, rat L6 whole cell, mouse heart tissue, mouse C2C12 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CDK2 antibody was positively detected by IHC in:
human breast cancer tissue, human laryngeal squamous cell carcinomas tissue, human liver cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CDK2 antibody was positively detected by FC in:
ANA-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Monkey
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Cdk2 Antibody Picoband® (monoclonal, 5B12D1)
View all CDK2 Antibodies
View all CDK2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
5B12D1
Isotype
Mouse IgG2a
Immunogen
E.coli-derived human Cdk2 recombinant protein (Position: E81-L298). Human Cdk2 shares 98.6% amino acid (aa) sequence identity with rat Cdk2.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
30 kDa
Calculated Molecular Weight
33.9 kDa
Background of CDK2
CDK2, Cyclin-Dependent Kinase2, is also known as P33. The CDK2 protein was highly homologous to p34(CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34(cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34(cdc2)/cyclin A. The gene for p33 shares 65% sequence identity with p34(cdc2). P33(cdk2) plays a unique role in cell cycle regulation of vertebrate cells.
Cite This Product
Anti-Cdk2 Antibody Picoband® (monoclonal, 5B12D1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00166-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Dog
100%
High Homology
Rat
99%
High Homology
Rabbit
99%
High Homology
Cow
99%
High Homology
Sheep
99%
High Homology
Zebrafish
89%
Moderate Homology
Drosophila
63%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CDK2 antibody
Immunohistochemistry (IHC) protocol for CDK2 antibody
Flow Cytometry (FC) protocol for CDK2 antibody
Publications
Application:
Species:
Bioengineered platelet nanoplatform enables renal-targeted dexamethasone delivery for chronic nephritis therapy with dual anti-inflammatory/anti-fibrotic effects and minimized systemic toxicity
Published: 2025-06-07
Journal: Bioactive Materials
Application: WB
Impact Factor: 20.30
Species: Mouse
PMID: 40535704
A natural halogenated fluoride adenosine analog 5′-fluorodeoxy adenosine induced anticolon cancer activity in vivo and in vitro
Published: 2022-07-22
Journal: ENVIRONMENTAL TOXICOLOGY
Application: WB
Impact Factor: 4.11
Species: Mouse
PMID: 35866996
Effect of dietary N-carbamylglutamate on development of ovarian follicles via enhanced angiogenesis in the chicken
Published: 2019-12-30
Journal: POULTRY SCIENCE
Application: WB
Impact Factor: 2.03
Species: Chicken
PMID: 32416844
JNK/STAT signalling pathway is involved in fluoride-induced follicular developmental dysplasia in female mice
Published: 2018-06-12
Journal: CHEMOSPHERE
Application: IHC,WB
Impact Factor: 4.43
Species: Mouse
PMID: 29913403
Enterovirus 71 mediates cell cycle arrest in S phase through non-structural protein 3D
Published: 2015-02-06
Journal: CELL CYCLE
Application: WB
Impact Factor: 4.57
Species: Human
PMID: 25659038
The effects of urokinase-type plasminogen activator (uPA) on cell proliferation and phenotypic transformation of rat mesangial cells induced by high glucose
Published: 2013-11-28
Journal: DIABETES RESEARCH AND CLINICAL PRACTICE
Application: WB
Impact Factor: 2.74
Species: Rat
PMID: 24447806
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Product Promise
We promise Anti-Cdk2 Antibody Picoband® (monoclonal, 5B12D1) performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00166-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026