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- Table of Contents
CDK2 antibody (M00166) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Immunofluorescent analysis of Hela cells, using CDK2 Antibody .
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Anti-CDK2 Rabbit Monoclonal Antibody
CDK2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
CDK2 antibody was positively detected by WB in:
human Jurkat whole cell, human HepG2 whole cell, human U2OS whole cell, human K562 whole cell, rat L6 whole cell, rat C6 whole cell, mouse C2C12 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CDK2 antibody was positively detected by IHC in:
human beast carcinoma tissue, Rat heart tissue, Rat pancreas tissue, Human non-Hodgkin's lymphoma tissue, Human liver cancer tissue, Mouse testis tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
Human
Tested
Tested
Recommend
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-CDK2 Rabbit Monoclonal Antibody
View all CDK2 Antibodies
View all CDK2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cdk2
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
30 kDa
Calculated Molecular Weight
33.9 kDa
Cite This Product
Anti-CDK2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00166)
Protocols
Western Blot protocol for CDK2 antibody
Immunohistochemistry (IHC) protocol for CDK2 antibody
Publications
Application:
Species:
ERK promotes miR34a/ISOC1 signaling to enhance all trans retinoic acid-induced myeloid differentiation in acute promyelocytic leukemia
Published: 2026-06-20
Journal: JOURNAL OF BIOLOGICAL CHEMISTRY
Application: WB
Impact Factor: 4.10
Species: Human
PMID: 42323085
tRF-31-PS5P4PW3FJHP inhibits hypoxia-induced proliferation of pulmonary artery endothelial cells by regulating EDN1 splicing via binding to LSM4
Published: 2025-12-05
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 4.70
Species: Mouse
PMID: 41354297
Identification and Validation of Antidepressant Small Molecules Using Bioinformatics and Mouse Depression Models
Published: 2025-08-20
Journal: Drug Design Development and Therapy
Application: WB
Impact Factor: 5.10
Species: Mouse
PMID: 40859969
Review
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1 Review For Anti-CDK2 Rabbit Monoclonal Antibody
WB analysis using Anti-CDK2 antibody (M00166) in mouse hippocampal tissue showed a specific band at the expected size with low background, indicating good performance.
| SKU | M00166 |
|---|---|
| Application | Western Blot |
| Sample | mouse hippocampal tissue |
| Sample Processing Description | Total protein was extracted from the left hippocampus of normal mouse brain. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | CDK2 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:3000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in RT |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | CDK2 (cyclin-dependent kinase 2) plays a central role in driving the cell cycle and serves as a key node integrating proliferative signaling, DNA damage response, and chemotherapy stress. In this study, hippocampal tissues from two normal mouse brains were used to evaluate the performance of the CDK2 antibody. The results showed a specific band at the expected molecular weight with good signal quality, indicating reliable antibody performance. |
Yajun Qiao, Qinghai University
Verified customer
Submitted 2026-05-06
Product Promise
We promise Anti-CDK2 Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00166
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026