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- Table of Contents
CDK4 antibody (M00159) performs well in IP, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Immunofluorescent analysis of NIH/3T3 cells, using CDK4 Antibody.
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Anti-CDK4 Rabbit Monoclonal Antibody
CDK4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IF, ICC, WB |
Tested Applications
CDK4 antibody was positively detected by WB in:
human Hela whole cell, human HepG2 whole cell, human MCF-7 whole cell, human K562 whole cell, rat brain tissue, rat pancreas tissue, mouse brain tissue, mouse pancreas tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CDK4 antibody was positively detected by ICC/IF in:
NIH/3T3 cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IP
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-CDK4 Rabbit Monoclonal Antibody
View all CDK4 Antibodies
View all CDK4 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HOB-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CDK4
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
34 kDa
Calculated Molecular Weight
33.7 kDa
Cite This Product
Anti-CDK4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00159)
Protocols
Western Blot protocol for CDK4 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CDK4 antibody
Publications
Application:
Species:
ERK promotes miR34a/ISOC1 signaling to enhance all trans retinoic acid-induced myeloid differentiation in acute promyelocytic leukemia
Published: 2026-06-20
Journal: JOURNAL OF BIOLOGICAL CHEMISTRY
Application: WB
Impact Factor: 4.10
Species: Human
PMID: 42323085
Silencing LY6K Suppresses CD44+ EpCAM+ HCT116 Human Colon Cancer Stem Cells Growth: Insights from In Vitro and In Vivo Evidence
Published: 2024-12-12
Journal: CURRENT ISSUES IN MOLECULAR BIOLOGY
Application: WB
Impact Factor: 2.80
Species: Human
PMID: 39727968
Licoricidin combats gastric cancer by targeting the ICMT/Ras pathway in vitro and in vivo.
Published: 2022-10-13
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 5.99
Species: Human
PMID: 36339587
Review
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Product Promise
We promise Anti-CDK4 Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00159
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026