Anti-Cdk6 Antibody Picoband™

CDK6 antibody

Boster Bio Anti-Cdk6 Antibody Picoband™ catalog # A00358. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse. Cited in 5 publication(s).

Product Info Summary

SKU: A00358
Size: 100 μg/vial
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, IHC, WB

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Product Name

Anti-Cdk6 Antibody Picoband™

View all CDK6 Antibodies

SKU/Catalog Number

A00358

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cdk6 Antibody Picoband™ catalog # A00358. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cdk6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00358)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Cdk6 recombinant protein (Position: M1-A326).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00358 is reactive to CDK6 in Human, Mouse

Applications

A00358 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

36.938kDa

Background of CDK6

Cell division protein kinase 6, also called Plstire, is an enzyme that in humans is encoded by the CDK6 gene. The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. Radiation hybrid analysis and inclusion within a mapped clone place the CDK6 gene at 7q21. Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation and promotes G1/S transition. This gene also involved in initiation and maintenance of cell cycle exit during cell differentiation. It prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types. In addition, CDK6 plays a role in promoting the proliferation of beta-cells in pancreatic islets of Langerhans.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For CDK6 (Source: Uniprot.org, NCBI)

Gene Name

CDK6

Full Name

Cyclin-dependent kinase 6

Weight

36.938kDa

Superfamily

protein kinase superfamily

Alternative Names

Cell division protein kinase 6; cyclin-dependent kinase 6; EC 2.7.11; EC 2.7.11.22; PLSTIREMGC59692; Serine/threonine-protein kinase PLSTIRE CDK6 MCPH12, PLSTIRE cyclin dependent kinase 6 cyclin-dependent kinase 6|cell division protein kinase 6|serine/threonine-protein kinase PLSTIRE

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CDK6, check out the CDK6 Infographic

CDK6 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CDK6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00358 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Long noncoding RNA NEAT1 promotes laryngeal squamous cell cancer through regulating miR-107/CDK6 pathway

Sun D,Zhao T,Long K,Wu M,Zhang Z.Triclosan down-regulates fatty acid synthase through microRNAs in HepG2 cells.Eur J Pharmacol .2021 Jun 15:174261.doi:10.1016/j.ejphar.2021.174261.Epub ahead of print.PMID:34144025.
Species: Human
A00358 usage in article: APP:WB, SAMPLE:HEPG2 CELL, DILUTION:1:400

TALENs-directed?knockout?of the?full-length?transcription?factor?Nrf1? that?represses?malignantbehaviour?of?human?hepatocellular?carcinoma?(HepG2)?cells

Long noncoding RNA NEAT1 promotes laryngeal squamous cell cancer through regulating miR-107/CDK6 pathway

Zhou R, Lu Z, Liu K, Guo J, Liu J, Zhou Y, Yang J, Mi M, Xu H. Curr Cancer Drug Targets. 2015;14(9):860-71. Platycodin D Induces Tumor Growth Arrest By Activating Foxo3A Expression In Prostate Cancer In Vitro And In Vivo.

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5 Customer Q&As for Anti-Cdk6 Antibody Picoband™

Question

We ordered your anti-Cdk6 antibody for WB on brain a few years ago. I am using mouse, and We want to use the antibody for IHC next. We want examining brain as well as tongue in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

I viewed the website and datasheets of our anti-Cdk6 antibody and I see that A00358 has been validated on mouse in both WB and IHC. Thus A00358 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-10-29

Question

I am interested in using your anti-Cdk6 antibody for oxidative stress induced senescence studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

Thanks for your inquiry. This A00358 anti-Cdk6 antibody is validated on human hela, hela whole cell lysates, k562 whole cell lysates, mouse sp20 whole cell lysates, intestinal cancer tissue, mammary cancer tissue. It is guaranteed to work for ELISA, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-05-31

Question

My colleagues were content with the WB result of your anti-Cdk6 antibody. However we have observed positive staining in tongue ruffle. using this antibody. Is that expected? Could you tell me where is CDK6 supposed to be expressed?

M. Anderson

Verified customer

Asked: 2018-03-02

Answer

From literature, tongue does express CDK6. Generally CDK6 expresses in cytoplasm. nucleus. cell projection, ruffle. Regarding which tissues have CDK6 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-03-02

Question

We are currently using anti-Cdk6 antibody A00358 for rat tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

S. Collins

Verified customer

Asked: 2014-11-04

Answer

The anti-Cdk6 antibody (A00358) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-11-04

Question

We have observed staining in rat tongue. Are there any suggestions? Is anti-Cdk6 antibody supposed to stain tongue positively?

G. Zhao

Verified customer

Asked: 2014-01-22

Answer

According to literature tongue does express CDK6. According to Uniprot.org, CDK6 is expressed in adrenal tissue, tongue, brain, leukemic t-cell, among other tissues. Regarding which tissues have CDK6 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2014-01-22

Order DetailsPrice
A00358

100μg

$370
A00358-10ug

10μg sample (liquid)

$99
A00358-Biotin

100 μg Biotin conjugated

$570
A00358-Cy3

100 μg Cy3 conjugated

$570
A00358-Dylight488

100 μg Dylight488 conjugated

$570
A00358-Dylight550

100 μg Dylight550 conjugated

$570
A00358-Dylight594

100 μg Dylight594 conjugated

$570
A00358-FITC

100 μg FITC conjugated

$570
A00358-HRP

100 μg HRP conjugated

$570
A00358-APC

100 μg APC conjugated

$670
A00358-PE

100 μg PE conjugated

$670
A00358-carrier-free

Carrier Free

$370

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A00358
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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