Anti-CDw75 (B-Cell Marker) Monoclonal Antibody

Product Name

Anti-CDw75 (B-Cell Marker) Monoclonal Antibody

View all ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibodies

SKU/Catalog Number

M03156

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CDw75 (B-Cell Marker) Monoclonal Antibody (Catalog # M03156). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CDw75 (B-Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03156)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: LN-1

Isotype

IgM, kappa

Immunogen

Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M03156 is reactive to ST6GAL1 in Human

Applications

M03156 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

46.605kDa

Background of ST6 Gal Sialyltransferase 1/ST6GAL1/CD75

Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This monoclonal antibody reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded normal human spleen tissue stained with Anti-CDw75 Mouse Monoclonal Antibody (LN-1).

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Surface staining of human lymphocytes with Anti-CDw75 Mouse Monoclonal Antibody (LN-1) (red) and isotype control (green). PPI negative population analyzed.

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Immunofluorescence analysis of MeOH-fixed HeLa cells labeled with Anti-CDw75 Mouse Monoclonal Antibody (LN-1) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Gene/Protein Information For ST6GAL1 (Source: Uniprot.org, NCBI)

Alternative Names

Alpha 2,6-ST; B-cell antigen CD75; beta-galactoside alpha-2,6-sialyltransferase 1; CD75; CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; EC 2.4.99.1; MGC48859; sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase); sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase); Sialyltransferase 1; SIAT1; SIAT1Alpha 2,6-ST 1; ST6 beta-galactosamide alpha-2,6-sialyltranferase 1; ST6Gal I; ST6GAL1; ST6GalI; ST6N ST6GAL1 SIAT1, ST6GalI, ST6N ST6 beta-galactoside alpha-2,6-sialyltransferase 1 beta-galactoside alpha-2,6-sialyltransferase 1|B-cell antigen CD75|CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase|CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1|ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 1|ST6 beta-galactosamide alpha-2,6-sialyltranferase 1|ST6Gal I|alpha 2,6-ST 1|sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase)

For more info on ST6GAL1, check out the ST6GAL1 Infographic

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In this infographic, you will see the following information for ST6GAL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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