radio
SKU MA1001
Size 100μg/vial
Reactivity Human
Clonality Monoclonal
Clone Number C3
Host Mouse
Ig Isotype Mouse IgG2a
Applications IHC

Overview

Product Name Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody
SKU/Catalog Number MA1001
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for AFP, alpha-fetoprotein (AFP) detection. Tested with IHC-P in Human. No cross reactivity with other proteins.
Cite This Product Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1001)
Host Mouse
Immunogen Human alpha-fetoprotein.
Reactivity Human

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, By Heat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Images And Assay Conditions

/antibody/ma1001 1 IHC anti afp alpha 1 fetoprotein antibody.jpg

Anti-AFP antibody (monoclonal), MA1001, IHC(P)
IHC(P): Human Liver Cancer Tissue

/antibody/ma1001 2 IHC anti afp alpha 1 fetoprotein antibody.jpg

Anti-AFP antibody (monoclonal), MA1001, IHC(P)
IHC(P): Human Hepatitis Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P02773
Gene Name Afp
Protein Name Alpha-fetoprotein
Tissue Specificity Plasma. .
Alternative Names Alpha-fetoprotein;Alpha-1-fetoprotein;Alpha-fetoglobulin;Afp;
Subcellular Localization Isoform 1: Secreted.
Molecular Weight 68386 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Binds estrogens, fatty acids and metals.
Background Alpha-fetoprotein(AFP) is a major plasma protein in the fetus, where it is produced by the yolk sac and liver. Direct confirmation of the assignment of the AFP gene to chromosome 4 by in situ hybridization was provided by Harper and Dugaiczyk(1983), who placed the gene in the q11-q22 region, the same region as the albumin gene. Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA.As the major fetal serum protein, Alpha-fetoprotein is not essential for embryonic development but is required for female fertility.

Order Product (MA1001)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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We promise all of our products perform as described in datasheets.

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Publications

Selective tropism of liver stem cells to hepatocellular carcinoma in vivo.
Adipose tissue-derived mesenchymal stem cells differentiated into hepatocyte-like cellsin vivoandin vitro
Extracellular regulated protein kinases 1/2 phosphorylation is required for hepatic differentiation of human umbilical cord-derived mesenchymal stem cells
The Viral Oncoprotein HBx of Hepatitis B virus Promotes the Growth of Hepatocellular Carcinoma through Cooperating with the Cellular Oncoprotein RMP
Continuous Passages Accelerate the Reprogramming of Mouse Induced Pluripotent Stem Cells
RPB5-mediating Protein Is Required for the Proliferation of Hepatocellular Carcinoma Cells
Mikulits, W., & Reichl, P. (2017). US Patent Application Publication No. US 2017/0146540 A1. Washington, DC: US Patent and Trademark Office.
Shan Zy, Wu Ys, Li X, Shen Xh, Wang Zd, Liu Zh, Shen Jl, Lei L. Cell Reprogram. 2014 Feb;16(1):77-83. Doi: 10.1089/Cell.2013.0067. Epub 2014 Jan 4. Continuous Passages Accelerate The Reprogramming Of Mouse Induced Pluripotent Stem Cells.
Su Z, Wu R, Tan Z, Li Y, Chen L, Luo J, Zhang M. Biochem Biophys Res Commun. 2010 Apr 9;394(3):616-22. Doi: 10.1016/J.Bbrc.2010.03.033. Epub 2010 Mar 7. Early Homing Behavior Of Stro-1- Mesenchyme-Like Cells Derived From Human Embryonic Stem Cells...
Yin L, Zhu Y, Yang J, Ni Y, Zhou Z, Chen Y, Wen L. Mol Med Rep. 2015 Mar;11(3):1722-32. Doi: 10.3892/Mmr.2014.2935. Epub 2014 Nov 13. Adipose Tissue-Derived Mesenchymal Stem Cells Differentiated Into Hepatocyte-Like Cells In Vivo And In Vitro.
Li Y, Wu Z, Liu Z. Analyst. 2015 Jun 21;140(12):4083-8. Doi: 10.1039/C5An00357A. Epub 2015 Apr 17. An Immune Sandwich Assay Of Carcinoembryonic Antigen Based On The Joint Use Of Upconversion Phosphors And Magnetic Beads.
Wu R, Gu B, Zhao X, Tan Z, Chen L, Zhu J, Zhang M. Hum Cell. 2013 Mar;26(1):19-27. Doi: 10.1007/S13577-011-0022-3. Epub 2011 Jun 15. Derivation Of Multipotent Nestin(+)/Cd271 (-)/Stro-1 (-) Mesenchymal-Like Precursors From Human Embryonic Stem Cel...
Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...
Qin Xg, Hua Z, Shuang W, Wang Yh, Cui Yd. Pharm Biol. 2010 Mar;48(3):275-81. Doi: 10.3109/13880200903104101. Effects Of Matrine On Hepg2 Cell Proliferation And Expression Of Tumor Relevant Proteins In Vitro.
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to afp antibody, alpha fetoprotein antibody, alpha-1-fetoprotein antibody, alpha-fetoprotein antibody