Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody

Boster Bio Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody catalog # MA1001. Tested in IHC applications. This antibody reacts with Human. Cited in 11 publication(s).

Product Info Summary

SKU: MA1001
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: IHC

Product Name

Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody

See all alpha-Fetoprotein/AFP products

SKU/Catalog Number

MA1001

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody catalog # MA1001. Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1001)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

C3

Isotype

Mouse IgG2a

Immunogen

Human alpha-fetoprotein.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1001 is reactive to Afp in Human

Applications

MA1001 is guaranteed for IHC Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, By Heat

Validation Images & Assay Conditions

Gene/Protein Information For Afp (Source: Uniprot.Org, NCBI)

Uniprot ID

P02773

Gene ID

24177

Gene Name

Afp

Full Name

Alpha-fetoprotein

Weight

68.386kDa

Superfamily

ALB/AFP/VDB family

Alternative Names

AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP Afp|alpha-fetoprotein|alpha-fetoprotein|alpha-1-fetoprotein|alpha-fetoglobulin|chloride intracellular channel 6

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Afp, check out the Afp Infographic

Afp infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Afp: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1001 has been cited in 11 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Selective tropism of liver stem cells to hepatocellular carcinoma in vivo.

Adipose tissue-derived mesenchymal stem cells differentiated into hepatocyte-like cellsin vivoandin vitro

Extracellular regulated protein kinases 1/2 phosphorylation is required for hepatic differentiation of human umbilical cord-derived mesenchymal stem cells

Shan Zy, Wu Ys, Li X, Shen Xh, Wang Zd, Liu Zh, Shen Jl, Lei L. Cell Reprogram. 2014 Feb;16(1):77-83. Doi: 10.1089/Cell.2013.0067. Epub 2014 Jan 4. Continuous Passages Accelerate The Reprogramming Of Mouse Induced Pluripotent Stem Cells.

Su Z, Wu R, Tan Z, Li Y, Chen L, Luo J, Zhang M. Biochem Biophys Res Commun. 2010 Apr 9;394(3):616-22. Doi: 10.1016/J.Bbrc.2010.03.033. Epub 2010 Mar 7. Early Homing Behavior Of Stro-1- Mesenchyme-Like Cells Derived From Human Embryonic Stem Cells...

Yin L, Zhu Y, Yang J, Ni Y, Zhou Z, Chen Y, Wen L. Mol Med Rep. 2015 Mar;11(3):1722-32. Doi: 10.3892/Mmr.2014.2935. Epub 2014 Nov 13. Adipose Tissue-Derived Mesenchymal Stem Cells Differentiated Into Hepatocyte-Like Cells In Vivo And In Vitro.

Li Y, Wu Z, Liu Z. Analyst. 2015 Jun 21;140(12):4083-8. Doi: 10.1039/C5An00357A. Epub 2015 Apr 17. An Immune Sandwich Assay Of Carcinoembryonic Antigen Based On The Joint Use Of Upconversion Phosphors And Magnetic Beads.

Wu R, Gu B, Zhao X, Tan Z, Chen L, Zhu J, Zhang M. Hum Cell. 2013 Mar;26(1):19-27. Doi: 10.1007/S13577-011-0022-3. Epub 2011 Jun 15. Derivation Of Multipotent Nestin(+)/Cd271 (-)/Stro-1 (-) Mesenchymal-Like Precursors From Human Embryonic Stem Cel...

Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...

Qin Xg, Hua Z, Shuang W, Wang Yh, Cui Yd. Pharm Biol. 2010 Mar;48(3):275-81. Doi: 10.3109/13880200903104101. Effects Of Matrine On Hepg2 Cell Proliferation And Expression Of Tumor Relevant Proteins In Vitro.

Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.

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4 Customer Q&As for Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody

Question

We are currently using anti-AFP/Alpha 1 Fetoprotein Monoclonal antibody MA1001 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-01

Answer

The anti-AFP/Alpha 1 Fetoprotein Monoclonal antibody (MA1001) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-01

Question

We have observed staining in human lung. What should we do? Is anti-AFP/Alpha 1 Fetoprotein Monoclonal antibody supposed to stain lung positively?

Verified Customer

Verified customer

Asked: 2019-05-14

Answer

Based on literature lung does express AFP. Based on Uniprot.org, AFP is expressed in embryo, heart, lung, brain cajal-retzius cell, among other tissues. Regarding which tissues have AFP expression, here are a few articles citing expression in various tissues:
Brain, and Cajal-Retzius cell, Pubmed ID: 6187626
Heart, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-05-14

Question

I was wanting to use using your anti-AFP/Alpha 1 Fetoprotein Monoclonal antibody for animal organ regeneration studies. Has this antibody been tested with western blotting on human hepatitis tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-03-15

Answer

We appreciate your inquiry. This MA1001 anti-AFP/Alpha 1 Fetoprotein Monoclonal antibody is tested on human hepatitis tissue, liver cancer tissue. It is guaranteed to work for IHC in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-03-15

Question

Our team were happy with the WB result of your anti-AFP/Alpha 1 Fetoprotein Monoclonal antibody. However we have seen positive staining in lung secreted. using this antibody. Is that expected? Could you tell me where is AFP supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-12-28

Answer

From literature, lung does express AFP. Generally AFP expresses in secreted. Regarding which tissues have AFP expression, here are a few articles citing expression in various tissues:
Brain, and Cajal-Retzius cell, Pubmed ID: 6187626
Heart, Pubmed ID: 14702039
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-12-28

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