Product Info Summary
SKU: | M00877-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CDX2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00877-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CDX2 Rabbit Monoclonal Antibody catalog # M00877-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDX2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00877-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFA-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CDX2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00877-1 is reactive to CDX2 in Human, Mouse, Rat
Applications
M00877-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
200 kDa
Calculated molecular weight
33.52kDa
Background of CDX2
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of CDX2 in (1) HEK293 cell lysate; (2) Colon cancer lysate.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human colon, using CDX2 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For CDX2 (Source: Uniprot.org, NCBI)
Gene Name
CDX2
Full Name
Homeobox protein CDX-2
Weight
33.52kDa
Superfamily
Caudal homeobox family
Alternative Names
caudal type homeo box transcription factor 2; caudal type homeobox 2; Caudal-type homeobox protein 2; CDX2; CDX3; CDX-3; CDX3caudal type homeobox transcription factor 2; homeobox protein CDX-2 CDX2 CDX-3/AS, CDX3, CDX2 caudal type homeobox 2 homeobox protein CDX-2|caudal type homeobox transcription factor 2|caudal-type homeobox protein 2|homeobox protein miniCDX2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDX2, check out the CDX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDX2 Rabbit Monoclonal Antibody (M00877-1)
Hello CJ!
M00877-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Overexpression of caudal-related homeobox transcription factor 2 inhibits the growth of transplanted colorectal tumors in nude mice
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-CDX2 Rabbit Monoclonal Antibody
Question
Will M00877-1 anti-CDX2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
As indicated on the product datasheet, M00877-1 anti-CDX2 Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-27
Question
I was wanting to use your anti-CDX2 Rabbit Monoclonal antibody for IHC for human mucosa of transverse colon on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human mucosa of transverse colon identification?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
It shows on the product datasheet, M00877-1 anti-CDX2 Rabbit Monoclonal antibody has been tested for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human mucosa of transverse colon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-04
Question
We need to test anti-CDX2 Rabbit Monoclonal antibody M00877-1 on human mucosa of transverse colon for research purposes, then I may be interested in using anti-CDX2 Rabbit Monoclonal antibody M00877-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-15
Answer
The products we sell, including anti-CDX2 Rabbit Monoclonal antibody M00877-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-15
Question
We are currently using anti-CDX2 Rabbit Monoclonal antibody M00877-1 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-23
Answer
The anti-CDX2 Rabbit Monoclonal antibody (M00877-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-23