Anti-CEACAM5/Cd66e Monoclonal Antibody

Boster Bio Anti-CEACAM5/Cd66e Monoclonal Antibody catalog # MA1023. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 3 publication(s).

Product Info Summary

SKU: MA1023
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: IHC, WB

Product Name

Anti-CEACAM5/Cd66e Monoclonal Antibody

See all CEACAM5/CD66e products

SKU/Catalog Number

MA1023

Size

100μg/vial

Form

lyophilized

Description

Boster Bio Anti-CEACAM5/Cd66e Monoclonal Antibody catalog # MA1023. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CEACAM5/Cd66e Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1023)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

C6G9

Isotype

Mouse IgG1

Immunogen

Carcinoembryonic antigen-related cell adhesion molecule 5 (CEA) isolated from a human colon adenocarcinoma cell line.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1023 is reactive to CEACAM5 in Human

Applications

MA1023 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-2μg/ml, Human, By Heat
Western blot, 0.5-1μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CEACAM5 (Source: Uniprot.Org, NCBI)

Uniprot ID

P06731

Gene ID

1048

Gene Name

CEACAM5

Full Name

Carcinoembryonic antigen-related cell adhesion molecule 5

Weight

76.795kDa

Superfamily

immunoglobulin superfamily

Alternative Names

Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e antigen; CD66e; CEA; CEACAM5; CEACAM-5; CEACD66e; DKFZp781M2392; Meconium antigen 100 CEACAM5 CD66e, CEA CEA cell adhesion molecule 5 carcinoembryonic antigen-related cell adhesion molecule 5|carcinoembryonic antigen related cell adhesion molecule 5|meconium antigen 100

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CEACAM5, check out the CEACAM5 Infographic

CEACAM5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CEACAM5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1023 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effects of Naringin on Proliferation and Osteogenic Differentiation of Human Periodontal Ligament Stem Cells In Vitro and In Vivo

Li H, Chen L, Zeng S, Li X, Zhang X, Lin C, Zhang M, Xie S, He Y, Shu S, Yang L, Tang S, Fu X. Exp Cell Res. 2015 Mar 1;332(1):67-77. Doi: 10.1016/J.Yexcr.2015.01.014. Epub 2015 Jan 30. Matrigel Basement Membrane Matrix Induces Eccrine Sweat Gland...

Hu C, Zheng J, Su X, Wang J, Wu W, Hu S. Anal Chem. 2013 Nov 5;85(21):10612-9. Doi: 10.1021/Ac4028005. Epub 2013 Oct 11. Ultrasensitive All-Carbon Photoelectrochemical Bioprobes For Zeptomole Immunosensing Of Tumor Markers By An Inexpensive Visibl...

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16 Customer Q&As for Anti-CEACAM5/Cd66e Monoclonal Antibody

Question

Is this MA1023 anti-CEACAM5/Cd66e Monoclonal antibody reactive to the isotypes of CEACAM1?

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

The immunogen of MA1023 anti-CEACAM5/Cd66e Monoclonal antibody is Carcinoembryonic antigen-related cell adhesion molecule 5 (CEA) isolated from a human colon adenocarcinoma cell line. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-24

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for monocyte using anti-CEACAM5/Cd66e Monoclonal antibody MA1023. Let me know if you need anything else.

M. Miller

Verified customer

Asked: 2020-02-28

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-28

Question

We are currently using anti-CEACAM5/Cd66e Monoclonal antibody MA1023 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

The anti-CEACAM5/Cd66e Monoclonal antibody (MA1023) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-04

Question

I am interested in to test anti-CEACAM5/Cd66e Monoclonal antibody MA1023 on human monocyte for research purposes, then I may be interested in using anti-CEACAM5/Cd66e Monoclonal antibody MA1023 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

The products we sell, including anti-CEACAM5/Cd66e Monoclonal antibody MA1023, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-07

Question

I see that the anti-CEACAM5/Cd66e Monoclonal antibody MA1023 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-16

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-16

Question

Please see the WB image, lot number and protocol we used for monocyte using anti-CEACAM5/Cd66e Monoclonal antibody MA1023. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-02

Question

Does anti-CEACAM5/Cd66e Monoclonal antibody MA1023 work on zebrafish IHC with colon adenocarcinoma?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

Our lab technicians have not validated anti-CEACAM5/Cd66e Monoclonal antibody MA1023 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-CEACAM5/Cd66e Monoclonal antibody MA1023 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish colon adenocarcinoma in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-15

Question

Do you have a BSA free version of anti-CEACAM5/Cd66e Monoclonal antibody MA1023 available?

F. Lewis

Verified customer

Asked: 2019-08-12

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CEACAM5/Cd66e Monoclonal antibody MA1023 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-12

Question

Will anti-CEACAM5/Cd66e Monoclonal antibody MA1023 work for IHC with monocyte?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

According to the expression profile of monocyte, CEACAM1 is highly expressed in monocyte. So, it is likely that anti-CEACAM5/Cd66e Monoclonal antibody MA1023 will work for IHC with monocyte.

Boster Scientific Support

Answered: 2019-08-05

Question

I have a question about product MA1023, anti-CEACAM5/Cd66e Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1023 anti-CEACAM5/Cd66e Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-18

Question

I was wanting to use your anti-CEACAM5/Cd66e Monoclonal antibody for IHC for human monocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human monocyte identification?

Verified Customer

Verified customer

Asked: 2019-02-18

Answer

As indicated on the product datasheet, MA1023 anti-CEACAM5/Cd66e Monoclonal antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human monocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-02-18

Question

We have observed staining in human leukocyte. Any tips? Is anti-CEACAM5/Cd66e Monoclonal antibody supposed to stain leukocyte positively?

D. Moore

Verified customer

Asked: 2018-07-12

Answer

Based on literature leukocyte does express CEACAM1. Based on Uniprot.org, CEACAM1 is expressed in colonic mucosa, leukocyte, monocyte, colon adenocarcinoma, skin, plasma, liver, among other tissues. Regarding which tissues have CEACAM1 expression, here are a few articles citing expression in various tissues:
Colon adenocarcinoma, Pubmed ID: 8018919, 15057824
Leukocyte, Pubmed ID: 2025273
Liver, Pubmed ID: 19159218, 24275569
Monocyte, Pubmed ID: 8423792
Plasma, Pubmed ID: 16335952
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-07-12

Question

Would MA1023 anti-CEACAM5/Cd66e Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

A. Edwards

Verified customer

Asked: 2018-03-27

Answer

As indicated on the product datasheet, MA1023 anti-CEACAM5/Cd66e Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-03-27

Question

We were content with the WB result of your anti-CEACAM5/Cd66e Monoclonal antibody. However we have observed positive staining in colon adenocarcinoma isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is CEACAM1 supposed to be expressed?

T. Anderson

Verified customer

Asked: 2017-11-27

Answer

Based on literature, colon adenocarcinoma does express CEACAM1. Generally CEACAM1 expresses in isoform 1: cell membrane, isoform 2: secreted, isoform 3: secreted, isoform 4: secreted, isoform 5: cell membrane. Regarding which tissues have CEACAM1 expression, here are a few articles citing expression in various tissues:
Colon adenocarcinoma, Pubmed ID: 8018919, 15057824
Leukocyte, Pubmed ID: 2025273
Liver, Pubmed ID: 19159218, 24275569
Monocyte, Pubmed ID: 8423792
Plasma, Pubmed ID: 16335952
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-11-27

Question

Is a blocking peptide available for product anti-CEACAM5/Cd66e Monoclonal antibody (MA1023)?

S. Jha

Verified customer

Asked: 2017-02-07

Answer

We do provide the blocking peptide for product anti-CEACAM5/Cd66e Monoclonal antibody (MA1023). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-02-07

Question

We bought anti-CEACAM5/Cd66e Monoclonal antibody for IHC on skin a few years ago. I am using human, and We are going to use the antibody for WB next. My question regards examining skin as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

B. Edwards

Verified customer

Asked: 2013-01-17

Answer

I have checked the website and datasheets of our anti-CEACAM5/Cd66e Monoclonal antibody and it appears that MA1023 has been tested on human in both IHC and WB. Thus MA1023 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-01-17

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