Anti-CEACAM5/Cd66e Monoclonal Antibody
|Ig Isotype||Mouse IgG1|
|Product Name||Anti-CEACAM5/Cd66e Monoclonal Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for CEACAM5/Cd66e detection. Tested with WB, IHC-P in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-CEACAM5/Cd66e Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1023)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Carcinoembryonic antigen-related cell adhesion molecule 5 (CEA) isolated from a human colon adenocarcinoma cell line.|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-2μg/ml, Human, By Heat
Western blot, 0.5-1μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
Images And Assay Conditions
Figure 1. Western blot analysis of CEACAM5/Cd66e using anti- CEACAM5/Cd66e antibody (MA1023).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Recombinant Human CEACAM5/Cd66e Protein 10ng
Lane 2; Recombinant Human CEACAM5/Cd66e Protein 5ng
Lane 3: Recombinant Human CEACAM5/Cd66e Protein 2.5ng
Lane 4: Recombinant Human CEACAM5/Cd66e Protein 1.25ng
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- CEACAM5/Cd66e antigen affinity purified monoclonal antibody (Catalog # MA1023) at 0.5 Î¼g/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
Figure 2. IHC analysis of CEACAM5/Cd66e using anti-CEACAM5/Cd66e antibody (MA1023).
CEACAM5/Cd66e was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1Î¼g/ml mouse anti-CEACAM5/Cd66e Antibody (MA1023) overnight at 4Â°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Protein Target Info (Source: Uniprot.org)
|Protein Name||Carcinoembryonic antigen-related cell adhesion molecule 5|
|Tissue Specificity||Expressed in columnar epithelial and goblet cells of the colon (at protein level) (PubMed:10436421). Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.|
|Alternative Names||Carcinoembryonic antigen-related cell adhesion molecule 5; Carcinoembryonic antigen; CEA; Meconium antigen 100; CD66e; CEACAM5|
|Subcellular Localization||Cell membrane|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:2803308, PubMed:10910050, PubMed:10864933). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells|
*You can search these to find other products in these research areas.
|Background||Carcinoembryonic antigen is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.|
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact firstname.lastname@example.org
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to email@example.com.
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to cea antibody, carcinoembryonic antigen antibody