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Product Info Summary
| SKU: | M01100-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-CEBP Beta Rabbit Monoclonal Antibody
SKU/Catalog Number
M01100-1
BM3970 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CEBP Beta Rabbit Monoclonal Antibody catalog # M01100-1. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CEBP Beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01100-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CEBP Beta
Reactive Species
M01100-1 is reactive to CEBPB in Human, Mouse, Rat
Observed Molecular Weight
33 kDa
Calculated molecular weight
36.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01100-1 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
FC 1:50
Positive Control
WB: PC-12 cell, Human spleen
Validation Images & Assay Conditions
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Western blot analysis of CEBP Beta in (1) PC-12 cell lysate; (2) Human spleen lysate.
Specific Publications For Anti-CEBP Beta Rabbit Monoclonal Antibody (M01100-1)
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Customer Q&As
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3 Customer Q&As for Anti-CEBP Beta Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-CEBP Beta Rabbit Monoclonal antibody for IP for mouse muscle skin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse muscle skin identification?
G. Parker
Verified customer
Asked: 2019-11-05
Answer
You can see on the product datasheet, M01100-1 anti-CEBP Beta Rabbit Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse muscle skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-05
Question
I have a question about product M01100-1, anti-CEBP Beta Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01100-1 anti-CEBP Beta Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-02
Question
Does M01100-1 anti-CEBP Beta Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
As indicated on the product datasheet, M01100-1 anti-CEBP Beta Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-10
