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Product Info Summary
| SKU: | A08747 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Cell adhesion molecule 3 CADM3 Antibody
SKU/Catalog Number
A08747
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Cell adhesion molecule 3 CADM3 Antibody (Catalog # A08747). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CADM3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Cell adhesion molecule 3 CADM3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08747)
Host
Rabbit
Contents
CADM3 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CADM3 antibody was raised against a 16 amino acid peptide near the center of human CADM3. The immunogen is located within amino acids 220 - 270 of CADM3.
Cross-reactivity
CADM3 antibody is human, mouse and rat reactive. At least three isoforms are known to exist; this antibody will only detect the two longest isoforms. CADM3 antibody is predicted to not cross-react with other members of the CADM protein family.
Reactive Species
A08747 is reactive to CADM3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
43.3 kDa
Background of CADM3
Cell adhesion molecule 3 (CADM3) is a member of a family of immunoglobulin-like, calcium-independent cell-cell adhesion molecules (1,2). CADM3 localizes to non-junctional contact sites of presynaptic nerve terminals, axons and glia cell processes and is thought to play an important role in the formation of synapses, axon bundles and myelinated axons (3). Recently, CADM3 has been shown to suppress the growth and tumorigenic ability of colon cancer cells, indicating that it can act as a tumor suppressor (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08747 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CADM3 antibody can be used for detection of CADM3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CADM3 in human brain tissue lysate with CADM3 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of CADM3 in mouse brain tissue with CADM3 antibody at 5 μg/ml.
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Immunofluorescence of CADM3 in mouse brain tissue with CADM3 antibody at 20 μg/ml.
Specific Publications For Anti-Cell adhesion molecule 3 CADM3 Antibody (A08747)
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