Click image to see more details
Product Info Summary
| SKU: | A07204-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC |
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Product info
Product Name
Anti-CELSR3/Flamingo Homolog 1 Antibody
SKU/Catalog Number
A07204-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CELSR3/Flamingo Homolog 1 Antibody (Catalog# A07204-1). Tested in ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CELSR3/Flamingo Homolog 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07204-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CELSR3. Immunogen sequence location: 3195 - 3244
Reactive Species
A07204-1 is reactive to CELSR3 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
358.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07204-1 is guaranteed for ELISA, IF, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ICC/IF 1:200 - 1:1000.
ELISA: 1:40000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of A549 cells, using CELSR3 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CELSR3/Flamingo Homolog 1 Antibody (A07204-1)
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Customer Q&As
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5 Customer Q&As for Anti-CELSR3/Flamingo Homolog 1 Antibody
Question
We are currently using anti-CELSR3/Flamingo Homolog 1 antibody A07204-1 for rat tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The anti-CELSR3/Flamingo Homolog 1 antibody (A07204-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-09
Question
I was wanting to use your anti-CELSR3/Flamingo Homolog 1 antibody for IF for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
It shows on the product datasheet, A07204-1 anti-CELSR3/Flamingo Homolog 1 antibody has been validated for IF on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-19
Question
Would A07204-1 anti-CELSR3/Flamingo Homolog 1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-03
Answer
As indicated on the product datasheet, A07204-1 anti-CELSR3/Flamingo Homolog 1 antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-03
Question
Will anti-CELSR3/Flamingo Homolog 1 antibody A07204-1 work for IF with brain?
Verified Customer
Verified customer
Asked: 2017-08-18
Answer
According to the expression profile of brain, CELSR3 is highly expressed in brain. So, it is likely that anti-CELSR3/Flamingo Homolog 1 antibody A07204-1 will work for IF with brain.
Boster Scientific Support
Answered: 2017-08-18
Question
We are interested in to test anti-CELSR3/Flamingo Homolog 1 antibody A07204-1 on rat brain for research purposes, then I may be interested in using anti-CELSR3/Flamingo Homolog 1 antibody A07204-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Miller
Verified customer
Asked: 2013-01-09
Answer
The products we sell, including anti-CELSR3/Flamingo Homolog 1 antibody A07204-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-01-09
