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Product Info Summary
| SKU: | A11021 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Centromere protein O CENPO Antibody
SKU/Catalog Number
A11021
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Centromere protein O CENPO Antibody (Catalog # A11021). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CENPO antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Centromere protein O CENPO Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11021)
Host
Rabbit
Contents
CENPO Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CENPO antibody was raised against a 16 amino acid synthetic peptide from near the center of human CENPO. The immunogen is located within amino acids 170 - 220 of CENPO.
Reactive Species
A11021 is reactive to CENPO in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
33.8 kDa
Background of CENPO
Accurate chromosome segregation during mitosis requires a kinetochore to assemble correctly at the centromere of each chromatid and form a dynamic interface with the microtubules of the mitotic spindle. The kinetochore assembly includes the multisubunit CENP-H/I complex which can be divided into three functional classes based on phenotype analysis. One of these classes is referred to as the CENP-O class of proteins of which CENPO is the exemplar. These proteins form a stable complex with each other and are required for proper kinetochore function and for recovery from mitotic spindle damage. CENPO has also been shown to be an auto-antigen in a small population of anti-centromere antibodies- (ACA-) positive patients with sclerodoma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11021 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CENPO antibody can be used for detection of CENPO by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CENPO in mouse kidney tissue lysate with CENPO antibody at (A) 1 and (B) 2 μg/mL.
Specific Publications For Anti-Centromere protein O CENPO Antibody (A11021)
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