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Product Info Summary
| SKU: | A08211 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Centrosomal protein of 170 kDa CEP170 Antibody
SKU/Catalog Number
A08211
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Centrosomal protein of 170 kDa CEP170 Antibody catalog # A08211. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Centrosomal protein of 170 kDa CEP170 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08211)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CEP170. AA range:701-750
Reactive Species
A08211 is reactive to CEP170 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
175.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08211 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using CEP170 Polyclonal Antibody diluted at 1:1000
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Western Blot analysis of AD293 cells using CEP170 Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from HepG2 and Jurkat cells, using CEP170 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human testis tissue, using CEP170 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Centrosomal protein of 170 kDa CEP170 Antibody (A08211)
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4 Customer Q&As for Anti-Centrosomal protein of 170 kDa CEP170 Antibody
Question
Will anti-CEP170 antibody A08211 work on primate WB with corpus callosum?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
Our lab technicians have not validated anti-CEP170 antibody A08211 on primate. You can run a BLAST between primate and the immunogen sequence of anti-CEP170 antibody A08211 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate corpus callosum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-17
Question
I see that the anti-CEP170 antibody A08211 works with WB, what is the protocol used to produce the result images on the product page?
H. Johnson
Verified customer
Asked: 2020-02-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-02-12
Question
I have attached the WB image, lot number and protocol we used for leukemic t-cell using anti-CEP170 antibody A08211. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-12
Question
I have a question about product A08211, anti-CEP170 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Baker
Verified customer
Asked: 2017-03-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08211 anti-CEP170 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-03-22
