Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | A10734 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB, IHC-P-Leica |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CEPT1 Antibody (N-term)
SKU/Catalog Number
A10734
Size
80 µl
Description
Boster Bio Anti-CEPT1 Antibody (N-term) (Catalog # A10734). Tested in IHC-P-Leica, WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CEPT1 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10734)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CEPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of human CEPT1.
Reactive Species
A10734 is reactive to CEPT1 in Human, Mouse
Calculated molecular weight
46.6 kDa
Background of CEPT1
Cholinephosphotransferase catalyses the final step in the synthesis of phosphatidylcholine by the transfer of phosphocholine from CDP-choline to diacylglycerol. The synthesis of phosphatidylethanolamine by ethanolaminephosphotransferase occurs using an analogous reaction. This gene codes for a choline/ethanolaminephosphotransferase. The protein can synthesize either choline- or ethanolamine- containing phospholipids. Two alternatively spliced transcripts encoding the same isoform have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10734 is guaranteed for WB, IHC-P-Leica Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P-Leica: 1:500
Validation Images & Assay Conditions
Click image to see more details
Anti-CEPT1 Antibody (N-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
All lanes : Anti-CEPT1 Antibody (N-term) at 1:4000 dilution Lane 1: HL-60 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Human cerebellum tissue lysate Lane 4: SK-BR-3 whole cell lysate Lane 5: Mouse brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
All lanes : Anti-CEPT1 Antibody (N-term) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: Human cerebellum tissue lysate Lane 3: SK-BR-3 whole cell lysate Lane 4: Human placenta tissue lysate Lane 5: Mouse spleen tissue lysate Lane 6: Mouse brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human breast tissue using A10734 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using A10734 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Specific Publications For Anti-CEPT1 Antibody (N-term) (A10734)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CEPT1 Antibody (N-term)?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-CEPT1 Antibody (N-term)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
