Anti-Choline Acetyltransferase/CHAT Antibody Picoband™

Boster Bio Anti-Choline Acetyltransferase/CHAT Antibody Picoband™ catalog # A01192-3. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 7 publication(s).

Product Info Summary

SKU: A01192-3
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Choline Acetyltransferase/CHAT Antibody Picoband™

See all Choline Acetyltransferase/ChAT products

SKU/Catalog Number

A01192-3

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Choline Acetyltransferase/CHAT Antibody Picoband™ catalog # A01192-3. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Choline Acetyltransferase/CHAT Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01192-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human CHAT recombinant protein (Position: D446-R652). Human CHAT shares 88.4% and 87% amino acid (aa) sequence identity with mouse and rat CHAT, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

A01192-3 is reactive to CHAT in Human, Mouse, Rat

Applications

A01192-3 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human

Validation Images & Assay Conditions

Gene/Protein Information For CHAT (Source: Uniprot.Org, NCBI)

Uniprot ID

P28329

Gene ID

1103

Gene Name

CHAT

Full Name

Choline O-acetyltransferase

Weight

82.536kDa

Superfamily

carnitine/choline acetyltransferase family

Alternative Names

acetyl CoA:choline O-acetyltransferase; CHAT; CHOACTase; Choline acetylase; Choline Acetyltransferase; choline O-acetyltransferase; CMS1A; CMS1A2; EC 2.3.1; EC 2.3.1.6 CHAT CHOACTASE, CMS1A, CMS1A2, CMS6 choline O-acetyltransferase choline O-acetyltransferase|acetyl CoA:choline O-acetyltransferase|choline acetylase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CHAT, check out the CHAT Infographic

CHAT infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CHAT: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A01192-3 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer

Autonomic remodeling may be responsible for decreased incidence of aortic dissection in STZ-induced diabetic rats via down-regulation of matrix metalloprotease 2

Overexpression of NTRK1 Promotes Differentiation of Neural Stem Cells into Cholinergic Neurons

Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma

Yu H, Xia H, Tang Q, Xu H, Wei G, Chen Y, Dai X, Gong Q, Bi F. Sci Rep. 2017 Jan 19;7:40802. doi: 10.1038/srep40802. Acetylcholine acts through M3 muscarinic receptor to activate the EGFR signaling and promotes gastric cancer cell proliferation

Zhao D, Xu X, Pan L, Zhu W, Fu X, Guo L, Lu Q, Wang J. J Neuroinflammation. 2017 Dec 2;14(1):234. doi: 10.1186/s12974-017-1007-2. Pharmacologic activation of cholinergic alpha7 nicotinic receptors mitigates depressive-like behavior in a mouse mode...

Liu B, Deng C, Zhang Y, Zhang J. Neural Regen Res. 2012 Jul 5;7(19):1463-8. Doi: 10.3969/J.Issn.1673-5374.2012.19.003. Wnt3A Expression During The Differentiation Of Adipose-Derived Stem Cells Into Cholinergic Neurons.

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15 Customer Q&As for Anti-Choline Acetyltransferase/CHAT Antibody Picoband™

Question

My lab would like using your anti-Choline Acetyltransferase/CHAT antibody for myasthenic syndrome studies. Has this antibody been tested with western blotting on rat testis tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-25

Answer

We appreciate your inquiry. This A01192-3 anti-Choline Acetyltransferase/CHAT antibody is tested on rat testis tissue, mouse brain. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-25

Question

We have been able to see staining in rat placenta. What should we do? Is anti-Choline Acetyltransferase/CHAT antibody supposed to stain placenta positively?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

According to literature placenta does express CHAT. According to Uniprot.org, CHAT is expressed in placenta, spinal cord, lymphocyte, among other tissues. Regarding which tissues have CHAT expression, here are a few articles citing expression in various tissues:
Lymphocyte, Pubmed ID: 1784419
Placenta, Pubmed ID: 3183663
Spinal cord, Pubmed ID: 1337937

Boster Scientific Support

Answered: 2019-12-13

Question

Does anti-Choline Acetyltransferase/CHAT antibody A01192-3 work for IHC with placenta?

Verified Customer

Verified customer

Asked: 2019-09-04

Answer

According to the expression profile of placenta, CHAT is highly expressed in placenta. So, it is likely that anti-Choline Acetyltransferase/CHAT antibody A01192-3 will work for IHC with placenta.

Boster Scientific Support

Answered: 2019-09-04

Question

We ordered your anti-Choline Acetyltransferase/CHAT antibody for WB on placenta in a previous experiment. I am using human, and We intend to use the antibody for IHC next. I am looking for examining placenta as well as spinal cord in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-06-14

Answer

I looked at the website and datasheets of our anti-Choline Acetyltransferase/CHAT antibody and it seems that A01192-3 has been tested on human in both WB and IHC. Thus A01192-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-06-14

Question

I see that the anti-Choline Acetyltransferase/CHAT antibody A01192-3 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-03-21

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-03-21

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Choline Acetyltransferase/CHAT antibody A01192-3. Let me know if you need anything else.

M. Dhar

Verified customer

Asked: 2018-05-08

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-05-08

Question

I was wanting to use your anti-Choline Acetyltransferase/CHAT antibody for IHC for rat placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat placenta identification?

Verified Customer

Verified customer

Asked: 2018-01-08

Answer

It shows on the product datasheet, A01192-3 anti-Choline Acetyltransferase/CHAT antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat placenta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-08

Question

Is this A01192-3 anti-Choline Acetyltransferase/CHAT antibody reactive to the isotypes of CHAT?

Verified Customer

Verified customer

Asked: 2017-12-12

Answer

The immunogen of A01192-3 anti-Choline Acetyltransferase/CHAT antibody is E.coli-derived human CHAT recombinant protein (Position: D446-R652). Human CHAT shares 88.4% and 87% amino acid (aa) sequence identity with mouse and rat CHAT, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-12-12

Question

I have a question about product A01192-3, anti-Choline Acetyltransferase/CHAT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

L. Carter

Verified customer

Asked: 2017-01-12

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01192-3 anti-Choline Acetyltransferase/CHAT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-01-12

Question

We are currently using anti-Choline Acetyltransferase/CHAT antibody A01192-3 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?

C. Roberts

Verified customer

Asked: 2016-09-22

Answer

The anti-Choline Acetyltransferase/CHAT antibody (A01192-3) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-09-22

Question

Do you have a BSA free version of anti-Choline Acetyltransferase/CHAT antibody A01192-3 available?

K. Yang

Verified customer

Asked: 2016-07-08

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Choline Acetyltransferase/CHAT antibody A01192-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-07-08

Question

Would A01192-3 anti-Choline Acetyltransferase/CHAT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

L. Taylor

Verified customer

Asked: 2015-12-28

Answer

You can see on the product datasheet, A01192-3 anti-Choline Acetyltransferase/CHAT antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-12-28

Question

I am interested in to test anti-Choline Acetyltransferase/CHAT antibody A01192-3 on rat placenta for research purposes, then I may be interested in using anti-Choline Acetyltransferase/CHAT antibody A01192-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

S. Baker

Verified customer

Asked: 2015-04-21

Answer

The products we sell, including anti-Choline Acetyltransferase/CHAT antibody A01192-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-04-21

Question

Is a blocking peptide available for product anti-Choline Acetyltransferase/CHAT antibody (A01192-3)?

D. Parker

Verified customer

Asked: 2015-01-13

Answer

We do provide the blocking peptide for product anti-Choline Acetyltransferase/CHAT antibody (A01192-3). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-01-13

Question

See below the WB image, lot number and protocol we used for placenta using anti-Choline Acetyltransferase/CHAT antibody A01192-3. Please let me know if you require anything else.

J. Williams

Verified customer

Asked: 2013-07-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-07-11

Conjugation

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A01192-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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