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Product Info Summary
| SKU: | M01192 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal Antibody
SKU/Catalog Number
M01192
BM5100 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal Antibody catalog # M01192. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01192)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABBO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CHAT
Reactive Species
M01192 is reactive to CHAT in Human, Mouse, Rat
Observed Molecular Weight
70, 82 kDa
Calculated molecular weight
82.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01192 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of CHAT expression in SH-SY5Y cell lysate.
Specific Publications For Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal Antibody (M01192)
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Customer Q&As
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8 Customer Q&As for Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal Antibody
Question
I was wanting to use using your anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal antibody for 6 studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
Thanks for your inquiry. This M01192 anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal antibody is validated on mouse brain. It is guaranteed to work for Flow Cytometry, IP, IF, IHC-P, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-10
Question
We have been able to see staining in rat lymphocyte. Are there any suggestions? Is anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal antibody supposed to stain lymphocyte positively?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
According to literature lymphocyte does express CHAT. According to Uniprot.org, CHAT is expressed in placenta, spinal cord, lymphocyte, among other tissues. Regarding which tissues have CHAT expression, here are a few articles citing expression in various tissues:
Lymphocyte, Pubmed ID: 1784419
Placenta, Pubmed ID: 3183663
Spinal cord, Pubmed ID: 1337937
Boster Scientific Support
Answered: 2020-02-17
Question
What was the positive control used when testing M01192 with Flow Cytometry?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
The positive control used for the Flow Cytometry test is SH-SY5Y cells labeling Choline Acetyltransferase.
Boster Scientific Support
Answered: 2020-01-06
Question
We have tried in the past anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal antibody for ICC on placenta a few years ago. I am using rat, and We intend to use the antibody for IP next. We want examining placenta as well as lymphocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
I viewed the website and datasheets of our anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal antibody and I see that M01192 has been validated on rat in both ICC and IP. Thus M01192 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-21
Question
A customer inquired if M01192 (Abways: CY6832) is suitable for FACS. There seems to be some discrepancies between the "Applications" field and the "Product description" for M01192: https://www.bosterbio.com/anti-chat-rabbit-monoclonal-antibody-m01192-boster.html?options=cart Could you help confirm with Abways?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
M01192 (Abways: CY6832) is suitable for FACS. We have fixed the application inconsistency. https://www.bosterbio.com/anti-chat-rabbit-monoclonal-antibody-m01192-boster.html?options=cart
Boster Scientific Support
Answered: 2019-11-05
Question
We are currently using anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal antibody M01192 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
The anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal antibody (M01192) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-07
Question
Has M01192 ever been listed as suitable for IHC-p?
Verified customer
Asked: 2019-06-13
Answer
The Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal Antibody (M01192) has not been listed as suitable for IHC-p.
Boster Scientific Support
Answered: 2019-06-13
Question
A customer wanted to know for M01192 (Abways CY6832), what was the positive control used for the Flow Cytometry test?
Verified Customer
Verified customer
Asked: 2015-11-09
Answer
The positive control used for the Flow Cytometry test is SH-SY5Y cells labeling Choline Acetyltransferase.
Boster Scientific Support
Answered: 2015-11-09
