Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal Antibody

Boster Bio Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal Antibody catalog # M01192. Tested in Flow Cytometry, IP, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: M01192
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC-P, ICC, WB

Product Name

Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal Antibody

See all Choline Acetyltransferase/ChAT products

SKU/Catalog Number

M01192

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal Antibody catalog # M01192. Tested in Flow Cytometry, IP, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01192)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABBO-3

Immunogen

A synthesized peptide derived from human CHAT

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01192 is reactive to CHAT in Human, Mouse, Rat

Applications

M01192 is guaranteed for Flow Cytometry, IP, IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC-P 1:50~1:200
ICC/IF 1:50~1:200
IP 1:50
Flow Cytometry 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CHAT (Source: Uniprot.Org, NCBI)

Uniprot ID

P28329

Gene ID

1103

Gene Name

CHAT

Full Name

Choline O-acetyltransferase

Weight

82.536kDa

Superfamily

carnitine/choline acetyltransferase family

Alternative Names

acetyl CoA:choline O-acetyltransferase; CHAT; CHOACTase; Choline acetylase; Choline Acetyltransferase; choline O-acetyltransferase; CMS1A; CMS1A2; EC 2.3.1; EC 2.3.1.6 CHAT CHOACTASE, CMS1A, CMS1A2, CMS6 choline O-acetyltransferase choline O-acetyltransferase|acetyl CoA:choline O-acetyltransferase|choline acetylase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CHAT, check out the CHAT Infographic

CHAT infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CHAT: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M01192 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer

Overexpression of NTRK1 Promotes Differentiation of Neural Stem Cells into Cholinergic Neurons

Yu H, Xia H, Tang Q, Xu H, Wei G, Chen Y, Dai X, Gong Q, Bi F. Sci Rep. 2017 Jan 19;7:40802. doi: 10.1038/srep40802. Acetylcholine acts through M3 muscarinic receptor to activate the EGFR signaling and promotes gastric cancer cell proliferation

Liu B, Deng C, Zhang Y, Zhang J. Neural Regen Res. 2012 Jul 5;7(19):1463-8. Doi: 10.3969/J.Issn.1673-5374.2012.19.003. Wnt3A Expression During The Differentiation Of Adipose-Derived Stem Cells Into Cholinergic Neurons.

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8 Customer Q&As for Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal Antibody

Question

I was wanting to use using your anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal antibody for 6 studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-10

Answer

Thanks for your inquiry. This M01192 anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal antibody is validated on mouse brain. It is guaranteed to work for Flow Cytometry, IP, IF, IHC-P, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-10

Question

We have been able to see staining in rat lymphocyte. Are there any suggestions? Is anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal antibody supposed to stain lymphocyte positively?

Verified Customer

Verified customer

Asked: 2020-02-17

Answer

According to literature lymphocyte does express CHAT. According to Uniprot.org, CHAT is expressed in placenta, spinal cord, lymphocyte, among other tissues. Regarding which tissues have CHAT expression, here are a few articles citing expression in various tissues:
Lymphocyte, Pubmed ID: 1784419
Placenta, Pubmed ID: 3183663
Spinal cord, Pubmed ID: 1337937

Boster Scientific Support

Answered: 2020-02-17

Question

What was the positive control used when testing M01192 with Flow Cytometry?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

The positive control used for the Flow Cytometry test is SH-SY5Y cells labeling Choline Acetyltransferase.

Boster Scientific Support

Answered: 2020-01-06

Question

We have tried in the past anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal antibody for ICC on placenta a few years ago. I am using rat, and We intend to use the antibody for IP next. We want examining placenta as well as lymphocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

I viewed the website and datasheets of our anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal antibody and I see that M01192 has been validated on rat in both ICC and IP. Thus M01192 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-21

Question

A customer inquired if M01192 (Abways: CY6832) is suitable for FACS. There seems to be some discrepancies between the "Applications" field and the "Product description" for M01192: https://www.bosterbio.com/anti-chat-rabbit-monoclonal-antibody-m01192-boster.html?options=cart Could you help confirm with Abways?

Verified Customer

Verified customer

Asked: 2019-11-05

Answer

M01192 (Abways: CY6832) is suitable for FACS. We have fixed the application inconsistency. https://www.bosterbio.com/anti-chat-rabbit-monoclonal-antibody-m01192-boster.html?options=cart

Boster Scientific Support

Answered: 2019-11-05

Question

We are currently using anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal antibody M01192 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

The anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal antibody (M01192) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-07

Question

Has M01192 ever been listed as suitable for IHC-p?

Verified customer

Asked: 2019-06-13

Answer

The Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal Antibody (M01192) has not been listed as suitable for IHC-p.

Boster Scientific Support

Answered: 2019-06-13

Question

A customer wanted to know for M01192 (Abways CY6832), what was the positive control used for the Flow Cytometry test?

Verified Customer

Verified customer

Asked: 2015-11-09

Answer

The positive control used for the Flow Cytometry test is SH-SY5Y cells labeling Choline Acetyltransferase.

Boster Scientific Support

Answered: 2015-11-09

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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M01192
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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