Product Info Summary
| SKU: | A14235 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Chondroadherin-like protein CHADL Antibody
SKU/Catalog Number
A14235
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Chondroadherin-like protein CHADL Antibody (Catalog # A14235). Tested in ELISA, WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CHADL antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Chondroadherin-like protein CHADL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14235)
Host
Rabbit
Contents
CHADL Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CHADL antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human CHADL. The immunogen is located within the last 50 amino acids of CHADL.
Cross-reactivity
CHADL antibody is human, mouse and rat reactive. CHADL antibody is predicted to not cross-react with Chondroadherin protein.
Reactive Species
A14235 is reactive to CHADL in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
82.4 kDa
Background of CHADL
The Chondroadherin-like (CHADL) protein, also known as SLRR4B, is homologous to the Chondroadherin protein and is expressed cartillaginous tissues (1). Like Chondroadherin, CHADL belongs to the small leucine-rich proteoglycan (SLRP) family and class IV subfamily and plays a significant role in the regulation of chondrocyte growth and proliferation (1,2). CHADL is thought to play a negative regualtory role in collagen fibrillogenesis and chondrocyte differentiation (1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14235 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CHADL antibody can be used for the detection of CHADL by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CHADL in mouse kidney tissue lysate with CHADL antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of CHADL in mouse skeletal muscle tissue with CHADL antibody at 5 μg/mL.
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Immunofluorescence of CHADL in mouse skeletal muscle tissue with CHADL antibody at 20 μg/mL.
Red: CHADL Antibody (A14235)
Blue: DAPI staining
Click image to see more details
Immunohistochemistry of CHADL in mouse skeletal muscle tissue with CHADL antibody at 5 μg/mL.
Specific Publications For Anti-Chondroadherin-like protein CHADL Antibody (A14235)
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