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Product Info Summary
| SKU: | M04073 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Chromogranin B CHGB Monoclonal Antibody
SKU/Catalog Number
M04073
BM5294 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Chromogranin B CHGB Monoclonal Antibody catalog # M04073. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Chromogranin B CHGB Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04073)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACFG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Chromogranin B Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
Reactive Species
M04073 is reactive to CHGB in Human, Mouse, Rat
Observed Molecular Weight
125 kDa
Calculated molecular weight
78.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04073 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: human SH-SY5Y whole cell lysates, human U251 whole cell lysates, human A549 whole cell lysates, human U2OS whole cell lysates, rat PC-12 whole cell lysates, mouse Neuro-2a whole cell lysates
Validation Images & Assay Conditions
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Western blot analysis of Chromogranin B/CHGB using anti-Chromogranin B/CHGB antibody (M04073).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chromogranin B/CHGB antigen affinity purified monoclonal antibody (M04073) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Chromogranin B/CHGB at approximately 125 kDa. The expected band size for Chromogranin B/CHGB is at 78 kDa.
Specific Publications For Anti-Chromogranin B CHGB Monoclonal Antibody (M04073)
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Customer Q&As
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4 Customer Q&As for Anti-Chromogranin B CHGB Monoclonal Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Chromogranin B Monoclonal antibody M04073. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-16
Question
We are currently using anti-Chromogranin B Monoclonal antibody M04073 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-31
Answer
The anti-Chromogranin B Monoclonal antibody (M04073) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-31
Question
I am looking for to test anti-Chromogranin B Monoclonal antibody M04073 on human liver for research purposes, then I may be interested in using anti-Chromogranin B Monoclonal antibody M04073 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Miller
Verified customer
Asked: 2015-10-09
Answer
The products we sell, including anti-Chromogranin B Monoclonal antibody M04073, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-10-09
Question
I was wanting to use your anti-Chromogranin B Monoclonal antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
J. Gonzalez
Verified customer
Asked: 2014-12-16
Answer
As indicated on the product datasheet, M04073 anti-Chromogranin B Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-16
