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Product Info Summary
| SKU: | A30610 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CHSY2 CHPF Antibody
SKU/Catalog Number
A30610
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CHSY2 CHPF Antibody (Catalog# A30610). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CHSY2 CHPF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30610)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CHSY2. Immunogen sequence location: 631-680
Reactive Species
A30610 is reactive to CHPF in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
85.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30610 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:40000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of MCF7 cells, using CHSY2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue, using CHSY2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from LOVO and K562 cells, using CHSY2 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CHSY2 CHPF Antibody (A30610)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CHSY2 CHPF Antibody
Question
Will anti-CHSY2 antibody A30610 work for WB with testis?
P. Mitchell
Verified customer
Asked: 2020-03-30
Answer
According to the expression profile of testis, CHPF is highly expressed in testis. So, it is likely that anti-CHSY2 antibody A30610 will work for WB with testis.
Boster Scientific Support
Answered: 2020-03-30
Question
We are currently using anti-CHSY2 antibody A30610 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
The anti-CHSY2 antibody (A30610) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-23
Question
I have a question about product A30610, anti-CHSY2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30610 anti-CHSY2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-11
Question
I was wanting to use your anti-CHSY2 antibody for WB for rat testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat testis identification?
S. Anderson
Verified customer
Asked: 2017-05-05
Answer
It shows on the product datasheet, A30610 anti-CHSY2 antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-05
Question
We are interested in to test anti-CHSY2 antibody A30610 on rat testis for research purposes, then I may be interested in using anti-CHSY2 antibody A30610 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Mangal
Verified customer
Asked: 2015-05-28
Answer
The products we sell, including anti-CHSY2 antibody A30610, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-05-28
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-CHSY2 antibody A30610. Let me know if you need anything else.
T. Krishna
Verified customer
Asked: 2014-02-25
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-02-25
