Anti-CIB1 Antibody Picoband™

Product Name

Anti-CIB1 Antibody Picoband™

View all CIB1 Antibodies

SKU/Catalog Number

PB9489

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CIB1 Antibody Picoband™ catalog # PB9489. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CIB1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9489)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CIB1 recombinant protein (Position: S6-L191). Human CIB1 shares 93.5% amino acid (aa) sequence identity with both mouse and rat CIB1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9489 is reactive to CIB1 in Human, Mouse, Rat

Applications

PB9489 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

27 kDa

Calculated molecular weight

21.703kDa

Background of CIB1

Calcium and integrin-binding protein 1 is a protein that in humans is encoded by the CIB1 gene. This gene encodes a member of the EF-hand domain-containing calcium-binding superfamily. And this protein interacts with many other proteins, including the platelet integrin alpha-IIb-beta-3, DNA-dependent protein kinase, presenilin-2, focal adhesion kinase, p21 activated kinase, and protein kinase D. Moreover, the encoded protein may be involved in cell survival and proliferation, and is associated with several disease states including cancer and Alzheimer's disease. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of CIB1 using anti-CIB1 antibody (PB9489).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Spleen Tissue Lysate at 50ug,
Lane 2: Rat Brain Tissue Lysate at 50ug,
Lane 3: Rat Thymus Tissue Lysate at 50ug,
Lane 4: Human Placenta Tissue Lysate at 50ug,
Lane 5: MCF-7 Whole Cell Lysate at 40ug,
Lane 6: NIH3T3 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIB1 antigen affinity purified polyclonal antibody (Catalog # PB9489) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CIB1 at approximately 27 kDa. The expected band size for CIB1 is at 27 kDa.

Gene/Protein Information For CIB1 (Source: Uniprot.org, NCBI)

Alternative Names

calcium and integrin binding 1 (calmyrin); Calcium- and integrin-binding protein; Calmyrin; CIB1; CIBcalcium and integrin binding protein; CIBP; DNA-dependent protein kinase interacting protein; DNA-PKcs-interacting protein; Kinase-interacting protein; KIP; KIP1; KIPcalcium and integrin binding, protein kinase interacting protein; PRKDCIP; SIP2-28; SIP2-28calcium and integrin-binding protein 1; Snk interacting protein 2-28; SNK-interacting protein 2-28 CIB1 CIB, CIBP, KIP1, PRKDCIP, SIP2-28 calcium and integrin binding 1 calcium and integrin-binding protein 1|DNA-PK interaction protein|DNA-PKcs-interacting protein|DNA-dependent protein kinase interacting protein|SNK-interacting protein 2-28|calcium and integrin binding 1 (calmyrin)|testicular secretory protein Li 9

For more info on CIB1, check out the CIB1 Infographic

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In this infographic, you will see the following information for CIB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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