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Product Info Summary
| SKU: | A02966 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-CITED2 Antibody
SKU/Catalog Number
A02966
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CITED2 Antibody (Catalog # A02966). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CITED2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CITED2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02966)
Host
Rabbit
Contents
CITED2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CITED2 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CITED2. The immunogen is located within the first 50 amino acids of CITED2.
Cross-reactivity
Multiple isoforms of CITED2 are known to exist.
Reactive Species
A02966 is reactive to CITED2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
28.5 kDa
Background of CITED2
CITED2 was initially identified as a potential transcriptional activator with significant homology to MSG1, a melanocyte-specific nuclear protein. CITED2 interacts with the histone acetyltransferase p300/CBP and acts as a coactivator to several DNA-binding transcription factors such as HIF-1 and AP-2. CITED2 also controls proliferation of mouse embryonic fibroblasts via the polycomb group genes Bmi-1 and Mel18 and the tumor suppressor Ink4a/Arf. CITED2 has also been found to be an essential regulator of adult hematopoietic stem cells (HSCs), with conditional deletion of CITED2 in the adult mouse resulting in the loss of HSCs, multilineage bone marrow failure and increased lethality.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02966 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CITED2 antibody can be used for detection of CITED2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CITED2 in Jurkat cell lysate with CITED2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of CITED2 in Jurkat cells with CITED2 antibody at 20 μg/mL.
Specific Publications For Anti-CITED2 Antibody (A02966)
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