Product Info Summary
| SKU: | A30464 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-CKS1 CKS1B Antibody
SKU/Catalog Number
A30464
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CKS1 CKS1B Antibody (Catalog# A30464). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CKS1 CKS1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30464)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CKS1. Immunogen sequence location: 10-59
Reactive Species
A30464 is reactive to CKS1B in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
9.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30464 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of various cells using Cks1 Polyclonal Antibody.
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Immunofluorescence analysis of HeLa cells, using CKS1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Jurkat cells, treated with serum 20% 15', using CKS1 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CKS1 CKS1B Antibody (A30464)
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Customer Q&As
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5 Customer Q&As for Anti-CKS1 CKS1B Antibody
Question
We are currently using anti-CKS1 antibody A30464 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
The anti-CKS1 antibody (A30464) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-25
Question
you antibody to test anti-CKS1 antibody A30464 on mouse muscle prostate for research purposes, then I may be interested in using anti-CKS1 antibody A30464 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
The products we sell, including anti-CKS1 antibody A30464, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-02
Question
I was wanting to use your anti-CKS1 antibody for IF for mouse muscle prostate on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse muscle prostate identification?
J. Dhar
Verified customer
Asked: 2019-03-01
Answer
You can see on the product datasheet, A30464 anti-CKS1 antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse muscle prostate in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-01
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for muscle prostate using anti-CKS1 antibody A30464. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-08
Question
I have a question about product A30464, anti-CKS1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Wu
Verified customer
Asked: 2015-06-10
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30464 anti-CKS1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-06-10

