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Product Info Summary
| SKU: | A03033 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Claudin 2 CLDN2 Antibody
SKU/Catalog Number
A03033
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Claudin 2 CLDN2 Antibody (Catalog # A03033). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Claudin 2 CLDN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03033)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Claudin 2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03033 is reactive to CLDN2 in Human, Mouse, Rat
Applications
A03033 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
24.549kDa
Background of CLDN2
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity By similarity.
Michele A. Battle, PNAS, May 2006; 103: 8419 - 8424.
Masahiko Itoh J. Cell Biol., Dec 1999; 147: 1351.
Salah Amasheh, J. Cell Sci., Dec 2002; 115: 4969.
Evemie Dub¨¦, Biol Reprod, Apr 2007; 10.1095/biolreprod.106.059246.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from HuvEc cells (Lane 2), using Claudin 2 antiobdy A03033. The lane on the left is treated with synthesized peptide.
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Western blot analysis of extracts from NIH/3T3 cells, using Claudin 2 antibody A03033.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Claudin 2 antibody A03033.
Protein Target Info & Infographic
Gene/Protein Information For CLDN2 (Source: Uniprot.org, NCBI)
Gene Name
CLDN2
Full Name
Claudin-2
Weight
24.549kDa
Superfamily
claudin family
Alternative Names
Claudin-2 CLDN2 claudin 2 claudin-2|SP82
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLDN2, check out the CLDN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLDN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Claudin 2 CLDN2 Antibody (A03033)
Hello CJ!
No publications found for A03033
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Claudin 2 CLDN2 Antibody
Question
We need using your anti-Claudin 2 antibody for calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules studies. Has this antibody been tested with western blotting on huvec cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
Thank you for your inquiry. This A03033 anti-Claudin 2 antibody is validated on huvec cells, breast carcinoma tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-18
Question
I was wanting to use your anti-Claudin 2 antibody for WB for mouse thalamus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse thalamus identification?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
You can see on the product datasheet, A03033 anti-Claudin 2 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse thalamus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-11
Question
Is this A03033 anti-Claudin 2 antibody reactive to the isotypes of CLDN2?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
The immunogen of A03033 anti-Claudin 2 antibody is Synthesized peptide derived from human Claudin 2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-25
Question
Our team were well pleased with the WB result of your anti-Claudin 2 antibody. However we have seen positive staining in teratocarcinoma cell junction using this antibody. Is that expected? Could you tell me where is CLDN2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-03-22
Answer
From what I have seen in literature, teratocarcinoma does express CLDN2. Generally CLDN2 expresses in cell junction, tight junction. Regarding which tissues have CLDN2 expression, here are a few articles citing expression in various tissues:
Colon epithelium, Pubmed ID: 11934881
Colon, and Skin, Pubmed ID: 15489334
Teratocarcinoma, Pubmed ID: 16303743
Thalamus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-03-22
Question
Does A03033 anti-Claudin 2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-18
Answer
It shows on the product datasheet, A03033 anti-Claudin 2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-18
Question
Can you help my question with product A03033, anti-Claudin 2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Miller
Verified customer
Asked: 2019-01-08
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03033 anti-Claudin 2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-08
Question
We are currently using anti-Claudin 2 antibody A03033 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
B. Krishna
Verified customer
Asked: 2018-05-15
Answer
The anti-Claudin 2 antibody (A03033) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-15
Question
We have been able to see staining in mouse colon skin. Are there any suggestions? Is anti-Claudin 2 antibody supposed to stain colon skin positively?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
From literature colon skin does express CLDN2. From Uniprot.org, CLDN2 is expressed in kidney epithelium, colon epithelium, thalamus, teratocarcinoma, colon skin, among other tissues. Regarding which tissues have CLDN2 expression, here are a few articles citing expression in various tissues:
Colon epithelium, Pubmed ID: 11934881
Colon, and Skin, Pubmed ID: 15489334
Teratocarcinoma, Pubmed ID: 16303743
Thalamus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-05-08
Question
Is a blocking peptide available for product anti-Claudin 2 antibody (A03033)?
H. Li
Verified customer
Asked: 2017-12-04
Answer
We do provide the blocking peptide for product anti-Claudin 2 antibody (A03033). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-04
Question
My lab would like to test anti-Claudin 2 antibody A03033 on mouse thalamus for research purposes, then I may be interested in using anti-Claudin 2 antibody A03033 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-24
Answer
The products we sell, including anti-Claudin 2 antibody A03033, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-24
Question
We bought anti-Claudin 2 antibody for IHC on colon skin a few years ago. I am using rat, and We want to use the antibody for WB next. My question regards examining colon skin as well as thalamus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
I looked at the website and datasheets of our anti-Claudin 2 antibody and I see that A03033 has been validated on rat in both IHC and WB. Thus A03033 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-10-12
Question
I see that the anti-Claudin 2 antibody A03033 works with WB, what is the protocol used to produce the result images on the product page?
R. Williams
Verified customer
Asked: 2017-09-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-26
Question
Do you have a BSA free version of anti-Claudin 2 antibody A03033 available?
Verified Customer
Verified customer
Asked: 2017-09-05
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Claudin 2 antibody A03033 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-05
Question
See below the WB image, lot number and protocol we used for thalamus using anti-Claudin 2 antibody A03033. Please let me know if you require anything else.
P. Gonzalez
Verified customer
Asked: 2016-01-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-01-20
Question
Will anti-Claudin 2 antibody A03033 work for WB with thalamus?
J. Moore
Verified customer
Asked: 2014-05-07
Answer
According to the expression profile of thalamus, CLDN2 is highly expressed in thalamus. So, it is likely that anti-Claudin 2 antibody A03033 will work for WB with thalamus.
Boster Scientific Support
Answered: 2014-05-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thalamus using anti-Claudin 2 antibody A03033. Let me know if you need anything else.
K. Huang
Verified customer
Asked: 2013-06-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-06-10
