Human TCN2/Transcobalamin-2 ELISA Kit PicoKine®

Transcobalamin II ELISA kit for Human

Human TCN2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TCN2 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK2038
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

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Product Name

Human TCN2/Transcobalamin-2 ELISA Kit PicoKine®

View all Transcobalamin II ELISA kits

SKU/Catalog Number

EK2038

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TCN2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TCN2 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human TCN2/Transcobalamin-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2038)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-W427

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2038 is reactive to TCN2 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK2038 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Transcobalamin II

TCN2 (Transcobalamin II; also TC-2 or TC) is a 42-44 kDa, monomeric, secreted member of the eukaryotic cobalamin transport family of molecules. This gene is mapped to 22q12.2. This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.

Kit Components

Catalog Number Description Quantity
EK2038-CAP Anti-Human TCN2 Pre-coated 96-well strip microplate 1
EK2038-ST Human TCN2 Standard 2 vials, 10 ng/tube
EK2038-DA Human TCN2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0150.1290.2230.4230.6891.0971.6762.111

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Transcobalamin II, Dilution ratio of 1:10, concentration in serum is 18-22.4ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TCN2/Transcobalamin-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)28511395792112311235755
Standard deviation17.3872.89457.5618.4574.11552.48
CV (%)6.1%6.4%7.9%6.6%6.6%9.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Transcobalamin II in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 128529231928629513.824.6%
Sample 21139124712121220120439.973.3%
Sample 357926174525960985830359.736.1%
*number of samples for each test n=16.

Gene/Protein Information For TCN2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P20062

Gene ID

6948

Gene Name

TCN2

Full Name

Transcobalamin-2

Weight

47.535kDa

Superfamily

eukaryotic cobalamin transport proteins family

Alternative Names

D22S676; D22S750; macrocytic anemia; TC; TC-2; TC2II; TCII; TCN2; Transcobalamin II; transcobalamin II; macrocytic anemia; transcobalamin IITC II; transcobalamin-2; Vitamin B12-binding Protein 2 TCN2 D22S676, D22S750, II, TC, TC II, TC-2, TC2, TCII transcobalamin 2 transcobalamin-2|macrocytic anemia|transcobalamin II|transcobalamin II; macrocytic anemia|vitamin B12-binding protein 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TCN2, check out the TCN2 Infographic

TCN2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TCN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK2038

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8 Customer Q&As for Human TCN2/Transcobalamin-2 ELISA Kit PicoKine®

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Transcobalamin II in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2019-12-30

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Transcobalamin II ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-12-30

Question

Q: can I use citrate plasma as samples in Human Transcobalamin II Picokine® ELISA Kit (Catalog # EK2038)?

B. Garcia

Verified customer

Asked: 2019-10-03

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Transcobalamin II causing degradation of its protein structure. Transcobalamin II may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Transcobalamin II ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-10-03

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-07-27

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-07-27

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-05-24

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

W. Wang

Verified customer

Asked: 2019-04-22

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-04-22

Question

Q: which procedure should I follow in order to thaw whole blood sample for Transcobalamin II ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-08-14

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Transcobalamin II for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-08-14

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Z. Hernandez

Verified customer

Asked: 2018-01-07

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-01-07

Question

Q: Can Transcobalamin II ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

S. Roberts

Verified customer

Asked: 2014-01-30

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2014-01-30

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