Anti-MIP-1 alpha CCL3 Antibody

Product Name

Anti-MIP-1 alpha CCL3 Antibody

View all CCL3 Antibodies

SKU/Catalog Number

A00405

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-MIP-1 alpha CCL3 Antibody (Catalog # A00405). Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening. (Ship at ambient temperature.)

Cite This Product

Anti-MIP-1 alpha CCL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00405)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse MIP-1α protein produced in E.coli.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00405 is reactive to CCL3 in Mouse

Observed Molecular Weight

42 kDa

Calculated molecular weight

10.3 kDa

Background of CCL3

Mouse MIP-1α, or Ccl3, of the intercrine beta (chemokine CC family) is a secreted cytokine with inflammatory, pyrogenic and chemokinetic properties. It is expressed in lung, spleen, and pancreas, and has a potent chemotactic activity for eosinophils. Ccl3 binding to a high-affinity receptor activates calcium release in neutrophils.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00405 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000
WB: 1:1000
This purified antibody has been tested in western blotting and is suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7-8 kDa in size corresponding to the mature 92 amino acid mouse MIP-1α/Ccl3 protein by western blotting in appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information CCL3

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