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Product Info Summary
| SKU: | M03683 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Claudin 4 Monoclonal Antibody
SKU/Catalog Number
M03683
BM5409 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Claudin 4 Monoclonal Antibody catalog # M03683. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Claudin 4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03683)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADEB-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Claudin 4
Reactive Species
M03683 is reactive to CLDN4 in Human
Observed Molecular Weight
18 kDa
Calculated molecular weight
22.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03683 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
WB: MCF7 cell
Validation Images & Assay Conditions
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Western blot analysis of Claudin 4 expression in MCF7 cell lysate.
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3 Customer Q&As for Anti-Claudin 4 Monoclonal Antibody
Question
I have a question about product M03683, anti-Claudin 4 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03683 anti-Claudin 4 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-27
Question
Does M03683 anti-Claudin 4 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-09-28
Answer
It shows on the product datasheet, M03683 anti-Claudin 4 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-28
Question
We are currently using anti-Claudin 4 Monoclonal antibody M03683 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
N. Edwards
Verified customer
Asked: 2016-01-01
Answer
The anti-Claudin 4 Monoclonal antibody (M03683) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-01-01
